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目的了解手足口病病原阳性检出率的相关因素。方法在2011年4月-2013年11月,通过监测哨点医院收集全市手足口病病例的咽拭子和血清标本1 522人份,并填写病例相关基本信息,采用荧光定量RT-PCR方法对标本进行核酸检测,分析肠道病毒阳性检出率的相关因素。结果 1 522人份标本中,肠道病毒阳性927份,其中EV71阳性379份,Cox A16阳性137份,其他肠道病毒阳性411份,总阳性率为60.9%。咽拭子标本的阳性率(61.37%)高于血清(28.81%),发病3 d内的标本阳性率(62.81%)高于3 d以上的标本(52.04%),岛内(66.71%)和近岛(65.69%)医院的标本阳性率高于岛外(44.47%)医院,随着发病年龄的增加,阳性检出率先升高后下降。结论标本类型、标本采集时间、医院地理位置、患者年龄与肠道病毒阳性检出率相关。
Objective To understand the related factors of the positive detection rate of HFMD pathogens. Methods From April 2011 to November 2013, a total of 1 522 swabs and serum samples from throat swabs and serum samples of hand, foot and mouth disease in the city were collected by monitoring sentinel hospitals, and the basic information of cases were filled in. The fluorescence quantitative RT-PCR method Specimens for nucleic acid detection, analysis of enterovirus positive detection rate of the relevant factors. Results Among the 1 522 human samples, 927 were positive for enterovirus, of which 379 were EV71 positive, 137 were Cox A16 positive and 411 were other enterovirus positive. The total positive rate was 60.9%. The positive rate (61.37%) was higher than that of the serum (28.81%) in the throat swab specimens, and the positive rate was 62.81% within 3 d after the onset of the disease (52.04% Nearly Island (65.69%) hospital specimens positive rate higher than the island (44.47%) hospitals, with the onset of age increased, the positive detection rate increased first and then decreased. Conclusion The type of specimen, the time of specimen collection, the geographical location of the hospital and the age of the patient are related to the positive detection rate of enterovirus.