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目的研究旋转磁场对放射损伤小鼠的造血保护作用。方法C57 BL6/J小鼠随机分为对照组和磁场组,各组小鼠分别接受致死(7．0Gy)及亚致死(6．5Gy)剂量Csγ射线照射,磁场组磁场处理30d,每天2次,每次1h,旋转磁场强度为0．6T。观察小鼠30d的存活率和存活期；检测放射后第7,10,14,21,28天的外周血细胞数；测定第12,16天骨髓单核细胞数(BMNC)及CFU-GM数；观察股骨骨髓病理切片、计数血管数并检测骨形态发生蛋白(BMPs)的表达水平。结果对照组和磁场组30d的平均存活率分别为10%和70%,平均存活期分别为(18±0．8)d和(27±0．7)d(P＜0．01)；磁场组的外周血细胞数、BMNC数、CFU-GM数均比对照组明显增加(P＜0．05)；骨髓病理检查显示,磁场可促进骨髓造血组织结构的修复,对照组和磁场组骨髓内的血管数分别为(2．2±0．23)根和(6．4±0．53)根(P＜0．01),磁场组的骨髓BMPs表达水平亦明显高于对照组。结论旋转磁场对放射损伤小鼠具有明显的保护作用,能促进放射损伤后造血细胞和造血微环境的修复。
Objective To study the hematopoietic protective effect of rotating magnetic field on radiation-injured mice. Methods C57BL6 / J mice were randomly divided into control group and magnetic field group. The mice in each group were exposed to lethal (7.0Gy) and sublethal (6.5Gy) doses of Csγ radiation. The magnetic field was treated with magnetic field for 30 days twice daily , Each 1h, rotating magnetic field strength of 0.6T. The survival and survival of mice were observed 30 days after transplantation. The number of peripheral blood cells was detected on the 7th, 10th, 14th, 21st and 28th days after radiation. The numbers of BMNC and CFU-GM were measured on the 12th and 16th day. The pathological sections of femur bone marrow were observed, the number of blood vessels was counted and the expression of bone morphogenetic protein (BMPs) was detected. Results The average survival rates of the control group and the magnetic field group for 30 days were 10% and 70%, respectively, and the average survival time was (18 ± 0.8) d and (27 ± 0.7) d respectively (P <0.01) The number of peripheral blood cells, BMNC and CFU-GM of the control group were significantly higher than those of the control group (P <0.05). The bone marrow biopsy showed that the magnetic field could promote the repair of bone marrow hematopoietic tissue, The number of blood vessels was (2.2 ± 0.23) roots and (6.4 ± 0.53) roots, respectively (P <0.01). The bone marrow BMPs expression level in the magnetic field group was also significantly higher than that in the control group. Conclusion The rotating magnetic field has a significant protective effect on radiation-injured mice and can promote the repair of hematopoietic cells and hematopoietic microenvironment after radiation injury.