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目的通过对江西省2007年-2012年急性弛缓性麻痹(AFP)病例实验室监测结果进行分析,为本省保持无脊髓灰质炎状态提供依据。方法采用L20B、RD细胞进行脊髓灰质炎病毒PV分离,脊髓灰质炎毒株通过微量中和实验分型后送至国家脊髓灰质炎实验室进行型内鉴定。结果 2007年-2012年共接收2 084份AFP病例粪便标本,其中有71份分离出脊髓灰质炎病毒、300份分离出非脊髓灰质炎肠道病毒(NPEV),分离率分别为3.41%、14.40%;分离出的脊髓灰质炎病毒中Ⅲ型脊髓灰质炎病毒的构成比最高,为51.19%;2010年-2012年脊髓灰质炎病毒的分离率显著低于2007年-2009年;分离出的脊髓灰质炎病毒均为疫苗相关株。结论江西省脊髓灰质炎病毒学监测处于较高水平,2007年-2012年急性弛缓性麻痹病例未发现脊髓灰质炎野病毒和VDPV,Ⅲ型脊髓灰质炎疫苗株病毒是分离株中的优势株,应继续加强监测。
Objective To analyze the laboratory monitoring results of acute flaccid paralysis (AFP) cases from 2007 to 2012 in Jiangxi Province, and to provide basis for the province to maintain polio-free status. Methods Poliovirus PV was isolated from L20B and RD cells. The poliovirus strain was sub-neutralized by micro-neutralization and then sent to the national polio laboratory for in-vivo identification. Results A total of 2 084 fecal samples of AFP were collected from 2007 to 2012, of which 71 isolated poliovirus and 300 isolated non-polio enterovirus (NPEV) with the isolation rates of 3.41% and 14.40 %; Poliovirus isolated poliovirus type III poliovirus composition of the highest ratio of 51.19%; 2010 - 2012 poliovirus isolation rate was significantly lower than 2007-2009; isolated spinal cord The plague viruses are vaccine-associated strains. Conclusions Surveillance of poliomyelitis in Jiangxi Province is at a high level. No poliovirus and VDPV were found in acute flaccid paralysis cases from 2007 to 2012. Poliovirus type III poliovirus was the predominant strain in the isolates, Monitoring should continue to be strengthened.