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本文选用克拉克结瘤与不结瘤等位基因系大豆为实验材料,对乙炔还原活体测定盆栽大豆共生固氮方法进行研究。测定了不同生育阶段盆栽大豆共生固氮量,并将乙炔还原活体测定方法与总氮差值法、乙炔还原离体测定方法和同位素稀释各方法相比较。经三年实验表明,乙炔还原活体测定共生固氮的方法是快速、灵敏、简便、可靠的。所测共生固氮值极显著,高于乙炔还原离体方法估测值;与同位素稀释各方法相比较,鼓粒以前,乙炔还原活体方法测定共生固氮值较高,鼓粒以后,活体方法估测值偏低。 乙炔还原活体测定共生固氮为短时间内筛选大豆——根瘤菌最佳共生体提供了简便、快速的方法。
In this paper, we selected Cox nodularis and non-nodulation allele-derived soybean as the experimental material to study the method of determination of symbiotic nitrogen fixation of potted soybeans by acetylene reduction in vivo. The symbiotic nitrogen fixation of potted soybean at different growth stages was measured, and the method of determination of acetylene reduction in vivo was compared with those of total nitrogen difference method, in vitro determination of acetylene reduction method and isotope dilution method. After three years of experiments show that acetylene reduction in vivo determination of symbiotic nitrogen fixation method is fast, sensitive, simple and reliable. The measured symbiotic nitrogen value was significantly higher than the estimated value of acetylene reduction in vitro method; compared with the isotope dilution method, before the drum grain, acetylene reduction in vivo determination of symbiotic nitrogen content is higher, after the drumming, in vivo method to estimate Low value. Acetylene reduction in vivo determination of symbiotic nitrogen fixation for a short period of time to select soybean - Rhizobium best symbionts provides a simple and rapid method.