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目的通过流感样病例监测,了解黄山市流感病原学分布情况,为临床诊断和预防控制提供依据。方法采集哨点医院流感样病例的咽拭子标本,采用RealTime RT-PCR方法检测流感病毒核酸并鉴定亚型。结果黄山市2009年8月到2012年12月哨点医院共采集到咽拭子标本1 800份,核酸检测阳性标本共422份,阳性率为23.44%。结论黄山市2009年主要以甲型H1N1流感为主,2010年主要以B型为主,2011年主要以B型和甲型H1N1流感为主,2012年主要以B型和季H3型流感为主,流感发病主要在冬春季节。
Objective To understand the distribution of influenza etiology in Huangshan City through the monitoring of influenza-like cases and provide the basis for clinical diagnosis and prevention and control. Methods Throat swab samples from sentinel hospital were collected. RealTime RT-PCR was used to detect influenza virus nucleic acid and identify subtypes. Results A total of 1 800 throat swabs were collected from sentinel hospitals in Huangshan from August 2009 to December 2012. 422 positive samples were detected by nucleic acid. The positive rate was 23.44%. Conclusions Huangshan City was mainly Influenza A (H1N1) in 2009, mainly B (2010), mainly B and A (H1N1) in 2012, and mainly Influenza B and H3 The incidence of influenza mainly in winter and spring.