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目的建立盐酸齐拉西酮血药浓度的测定方法。方法采用反相高效液相色谱法测定盐酸齐拉西酮血药浓度,色谱柱采用Kromasil C18(4.6 mm×250 mm,5μm),流动相:甲醇-乙腈-40 mmol.L-1乙酸铵溶液(用乙酸调节pH值至3.50±0.1)(100∶100∶155);流速:1.0 mL.min-1;内标:卡马西平;检测波长:254 nm。结果以盐酸齐拉西酮与内标物峰面积之比(Y)对盐酸齐拉西酮血清浓度(C)进行线性回归,得回归方程:C=213Y-85.5(r=0.996 2),表明本法在20.0~1 200μg.L-1内线性关系良好,平均加样回收率为97.4%。结论本法操作简便、结果准确,可以用于临床盐酸齐拉西酮血药浓度的测定。
Objective To establish a method for the determination of ziprasidone hydrochloride in plasma. Methods The plasma concentration of ziprasidone hydrochloride was determined by reversed-phase high performance liquid chromatography (HPLC). The chromatographic column was Kromasil C18 (4.6 mm × 250 mm, 5 μm). The mobile phase was methanol-acetonitrile-40 mmol·L- (Adjusted to pH 3.50 ± 0.1 with acetic acid) (100: 100: 155); flow rate: 1.0 mL.min-1; internal standard: carbamazepine; detection wavelength: 254 nm. Results The linear regression of ziprasidone hydrochloride serum concentration (C) with ziprasidone hydrochloride and internal standard peak area ratio (Y) showed that the regression equation was C = 213Y-85.5 (r = 0.996 2) The law in 20.0 ~ 1 200μg.L-1 within a good linear relationship, the average recovery was 97.4%. Conclusion This method is simple, accurate and can be used to determine the serum concentration of ziprasidone hydrochloride.