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目的观察灯盏花素(breviscapine,Bre)对高糖环境原代培养的近端小管上皮细胞(proximal tubule epithelial cells,PTEC)钠钾ATP酶(Na+,K+-ATPase)活性的影响。方法手工微分离法原代培养PTEC,MTT法检测不同浓度的灯盏花素作用72h对PTEC增殖的影响,以确定合适的灯盏花素实验浓度。将细胞分为5组:正常对照组(NC组)、高糖组(HG组)、小剂量灯盏花素组、中剂量灯盏花素组、大剂量灯盏花素组,每组设6个复孔,作用72h。液闪法测定PTEC钠钾ATP酶活性。结果HG组PTEC钠钾ATP酶活性显著高于NC组(P<0.01),小剂量组与HG组比较无统计学意义,中剂量组与HG组比较明显降低(P<0.05),大剂量组显著降低(P<0.01)。结论高糖环境下PTEC的钠钾ATP酶活性明显升高;灯盏花素对高糖环境原代PTEC钠钾ATP酶活性的升高有明显的抑制作用;其对糖尿病肾病(diabetic nephropathy,DN)的防治作用可能部分通过抑制PTEC钠钾ATP酶活性实现。
Objective To observe the effect of breviscapine (Bre) on the activity of sodium potassium ATPase (Na+, K+-ATPase) in primary tubule epithelial cells (PTEC) cultured in high glucose environment. Methods PTEC was cultured by manual microseparation. The effect of different concentrations of scutellarin on the proliferation of PTEC was detected by MTT assay to determine the appropriate experimental concentration of scutellarin. The cells were divided into 5 groups: normal control group (NC group), high glucose group (HG group), low-dose breviscapine group, medium-dose breviscapine group, and large-dose breviscapine group, each group having 6 complexes Hole, role 72h. The liquid flash method was used to determine the sodium and potassium ATPase activity of PTEC. Results The sodium potassium ATPase activity of PTEC in HG group was significantly higher than that in NC group (P<0.01). There was no significant difference between low dose group and HG group. The middle dose group was significantly lower than that in HG group (P<0.05). Significantly decreased (P<0.01). Conclusions The activity of sodium-potassium ATPase in PTEC is significantly increased under high glucose conditions; breviscapine has a significant inhibitory effect on the increase of sodium glucoside ATPase activity of primary PTEC in high-glucose environment; Diabetic nephropathy (DN) The prevention and control effects may be partially achieved by inhibiting the sodium potassium ATPase activity of PTEC.