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琼脂斜面母种,易失水、老化,保存时间较短,且在转接时有的琼脂块不萌发,有的长势较弱。为此,我们于1993年初试用麦粒培养基制试管母种,经三年多的实践证明,用麦粒生产试管母种具有诸多优点:原料价廉,操作简单,对菌种有复壮作用,菌丝萌发快且粗壮,抗老化,室温保藏一年左右也不会影响菌种质量。具体操作:称取干小麦1kg,加水煮至无白心(全张而不破皮),沥水后凉晒干至麦粒抓在手里不湿手为宜,装管至1/2,加棉塞,每公斤小麦可装150支试管,每10支扎成一捆用薄膜包住棉塞,高压灭菌1.5小时或常压灭菌6小时,自然冷却后即可用于接种。
Agar slant mother species, water loss, aging, preservation time is short, and in the transfer of some agar block does not germinate, and some weak growth. To this end, we tried wheat kernel culture tube test tube mother in early 1993, after more than three years of practice proved that the use of wheat kernel tube production has many advantages: cheap raw materials, simple operation, rejuvenation of bacteria, Mycelium germination fast and robust, anti-aging, room temperature preservation for about a year will not affect the quality of strains. Specific operations: Weigh dry wheat 1kg, add boiled until no white heart (all Zhang without broken skin), after the drain dry cool to dry in the hands of wheat is not wet hands appropriate, tube to 1/2, add cotton Plug, each kilogram of wheat can hold 150 test tubes, tied with a bundle of 10 bundles of tampons wrapped with a film, autoclaved 1.5 hours or atmospheric sterilization for 6 hours, after natural cooling can be used for inoculation.