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目的研究不同强度50Hz脉冲电磁场(Pulse electromagnetic fields,PEMFs)对小鼠成骨细胞系MC3T3-E1增殖与分化的影响。方法 MC3T3-E1传代培养后分成七组,分别采用0.0 m T、0.6 m T、1.2 m T、1.8 m T、2.4 m T、3.0 m T和3.6 m T 50 Hz脉冲电磁场处理,90 min/d。MTT法检测细胞增殖情况,成骨性分化检测处理3 d、6 d、9 d、12 d后ALP活性和首次处理12 h后BMP-2、Runx-2、OPG基因表达情况。结果 6种强度的脉冲电磁场均促进细胞增殖,其中0.6 m T的促增殖效应最强(P<0.01)。在成骨性诱导培养条件下,细胞内ALP活性随电磁场处理时间的延长而逐渐增加,第9 d达到峰值,之后开始回落。0.6 m T、3.0 m T和3.6 m T均能提高ALP活性,其中0.6 m T始终处于最高水平(P<0.01)。三种基因的表达水平在首次处理12 h,在0.6 m T、1.2 m T、3 m T和3.6 m T组均显著提高,但仍然是0.6 m T的增强效应最为明显。结论 0.6 m T 50 Hz脉冲电磁场具有最佳的促进MC3T3-E1细胞增殖和成骨性分化活性,这可能为采用脉冲电磁场治疗骨质疏松症提供了理想的治疗参数。
Objective To study the effects of different intensity 50 Hz pulsed electromagnetic fields (PEMFs) on the proliferation and differentiation of mouse osteoblastic cell line MC3T3-E1. Methods MC3T3-E1 cells were subcultured into seven groups and treated with pulsed electromagnetic fields of 0.0 m T, 0.6 m T, 1.2 m T, 1.8 m T, 2.4 m T, 3.0 m T and 3.6 m T 50 Hz, respectively. After 90 min / d . The cell proliferation was detected by MTT assay. The ALP activity at 3 d, 6 d, 9 d and 12 d after osteoblast differentiation assay and BMP-2, Runx-2 and OPG gene expression after the first treatment for 12 h were detected. Results Six kinds of pulsed electromagnetic fields all promoted cell proliferation, of which 0.6 m T had the strongest proliferative effect (P <0.01). Under osteogenic induction culture, the intracellular ALP activity gradually increased with the prolongation of the electromagnetic field treatment, reaching the peak on the 9th day and then began to decline. 0.6 m T, 3.0 m T and 3.6 m T increased ALP activity, of which 0.6 m T was always at the highest level (P <0.01). The expression levels of the three genes in the first treatment of 12 h, 0.6 m T, 1.2 m T, 3 m T and 3.6 m T group were significantly increased, but still 0.6 m T enhancement effect most obvious. Conclusion 0.6 m T 50 Hz pulsed electromagnetic field has the best promoting activity of proliferation and osteogenic differentiation of MC3T3-E1 cells, which may provide an ideal therapeutic parameter for the treatment of osteoporosis by pulsed electromagnetic field.