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目的探讨番茄红素联合α-生育酚对晶状体氧化损伤进行药物治疗的可行性。方法体外培养正常大鼠晶状体,按随机分组原则将大鼠晶状体分为以下8组:空白对照组(A),过氧化氢处理组(B),过氧化氢和不同浓度番茄红素(1μmol/L、5μmol/L、10μmol/L)处理组(C1、C2、C3),过氧化氢和不同浓度番茄红素(1μmol/l、5μmol/L、10μmol/L)联合α-生育酚处理组(D1、D2、D3)。紫外分光光度计测定晶状体组织的总抗氧化能力(T-AOC)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的变化。结果培养24 h结束后,不同浓度番茄红素处理组及不同浓度番茄红素联合α-生育酚用药组SOD、CAT活性和GSH、T-AOC含量均显著高于过氧化氢处理组,MDA含量均显著低于过氧化氢处理组,经统计学分析,差异有显著统计学意义(P<0.01),不同浓度番茄红素处理组以5μmol/l抑制晶状体氧化损伤的效果最明显;联合应用α-生育酚可以增强番茄红素的作用。不同浓度番茄红素处理组与联合用药组各小组间两两比较,1μmol/L番茄红素联合α-生育酚处理组与5μmol/L番茄红素处理组间没有显著性差别,其余各组间差异均有显著统计学意义(P<0.01)。结论番茄红素可以抑制过氧化氢诱导的晶状体混浊,番茄红素联合α-生育酚抑制试验性白内障的形成是通过其抗氧化作用发挥作用,在α-生育酚存在的条件下可以增强番茄红素的作用,使低浓度的番茄红素发挥更好的作用。番茄红素联合α-生育酚有望成为预防或治疗白内障的新药。
Objective To investigate the feasibility of lycopene combined with α-tocopherol for the treatment of lens oxidative damage. Methods Normal rat lens were cultured in vitro. According to the principle of randomization, the rats were divided into the following 8 groups: blank control group (A), hydrogen peroxide treatment group (B), hydrogen peroxide and different concentrations of lycopene (1μmol / (C1, C2 and C3), hydrogen peroxide and different concentration of lycopene (1μmol / l, 5μmol / L, 10μmol / L) and α-tocopherol treatment group D1, D2, D3). The total antioxidant capacity (T-AOC), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) Variety. Results At the end of 24 h, the contents of SOD, CAT, GSH and T-AOC were significantly higher in the groups treated with different concentrations of lycopene and the different concentrations of lycopene combined with α-tocopherol than those in the group treated with hydrogen peroxide. The content of MDA Were significantly lower than that of hydrogen peroxide treatment group, the statistical analysis, the difference was statistically significant (P <0.01), different concentrations of lycopene treatment group 5μmol / l inhibition of lens oxidation damage the most obvious effect; combination of α Tocopherol can enhance the role of lycopene. There was no significant difference between the groups treated with 1μmol / L lycopene and α-tocopherol and the group treated with 5μmol / L lycopene with different concentrations of lycopene and the combined treatment groups. The other groups The differences were statistically significant (P <0.01). Conclusions Lycopene can inhibit hydrogen peroxide-induced lens opacity. Lycopene combined with α-tocopherol inhibits the formation of experimental cataract through its anti-oxidative effect. In the presence of α-tocopherol, tomato lycopene The role of prime, so that low concentrations of lycopene play a better role. Lycopene combined with alpha-tocopherol is expected to become a new drug to prevent or treat cataract.