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1.怎样自制母种、原种、栽培种? (1) 母种:称小麦5斤,用水淘洗干净,加水煮沸约20分钟过滤(过滤后的麦粒可用来制作原种培养基)。取上层澄清液1,000毫升,微火烧开,加入琼脂20克,搅拌溶化。再加入蚕白胨5克、磷酸二氢钾3克、硫酸镁1.5克,葡萄糖20克,维生素B_110毫克。趁热分装于试管中,置高压锅内,以每平方厘米1.2公斤的压力灭菌30分钟,取出后摆成斜
1. How to make mother species, original species and cultivated species? (1) Mother species: Weigh 5 kg of wheat and wash it with water, then add water and boil for about 20 minutes to filter (the filtered wheat grains can be used to make original culture medium). Take the supernatant 1,000 ml, micro-fire boiled, add 20 grams of agar, stirring and melting. Then add silkworm peptone 5 grams, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 20 grams of glucose, vitamin B_110 mg. While still hot in the test tube, set the pressure cooker to 1.2 kg per square centimeter of pressure sterilization 30 minutes, remove the ramp