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目的研究蛋白酶sirtuin 1在牙周膜干细胞骨向分化中的作用。方法筛选人因正畸而拔除的前磨牙，获取牙周膜组织做细胞培养；将细胞按照实验组、对照组进行分组。实验组I：加入Sirtuin1激活剂白藜芦醇使其工作浓度为1 mM,5mM,10mM,实验组I：加入Sirtuin1抑制剂烟酰胺使其终末工作浓度为1 mM,5mM,10mM，对照组为空白对照。获取培养后细胞做蛋白酶Sirtuin2和各种细胞骨向分化标志物检测：West-blotting检测Sirtuin1和各种骨向分化标志物蛋白的表达；RT-PCR检测骨向分化标志物ALP，骨桥蛋白，骨钙蛋白和骨涎蛋白。结果通过检测细胞的蛋白酶Sirtuin1和骨向分化标志物ALP，骨桥蛋白，骨钙蛋白和骨涎蛋白的mRNA，表明随着加入白芦藜醇的剂量的差异而出现梯度的上调；而随着烟酰胺的浓度的升高而出现梯度的下调。结论sirtuin 1可以有效的促进牙周膜干细胞骨向分化，从而促进牙槽骨再生修复重建，具有良好的临床应用前景。“,” Objective: To identify the role of SIRT1 in osteogenic differentiation of periodontal ligament stem cels. Method: we investigated the effects of overexpressing and underexpressing SIRT1 on the differentiation of periodontal ligament stem cels, Expression of SIRT1 and osteoblastic differentiation markers was assessed by RT-PCR, and western blotting. Result: Activation of SIRT1 using resveratrol stimulated osteoblastic differentiation in a dose-dependent manner, as assessed by the expression of mRNAs encoding alkaline phosphatase, osteopontin, osteocalcin, osterix and Runx2, and induced calcium deposition. In contrast, inhibition of SIRT1 using nicotinamide suppressed mineralization and the expression of osteoblast marker mRNAs.. Conclusion: It is verified that SIRT1 is a potent regulator of differentiation of human periodontal ligament cells and may have clinical implications for periodontal bone regeneration.