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目的 :研究耐力训练对大鼠力竭运动后淋巴细胞凋亡的影响 ,并分析其机制。方法 :将SD大鼠随机分为安静组、力竭运动组、训练 +力竭运动组。训练 +力竭运动组进行 3周游泳耐力训练后 ,除安静组外 ,全部大鼠负重 4 %进行力竭游泳 ,比较运动后即刻大鼠脾细胞、胸腺细胞及外周血液淋巴细胞凋亡率 ,细胞内Ca2 +浓度及血清皮质醇 (C)浓度。结果 :训练 +力竭运动组大鼠力竭游泳时间明显长于力竭运动组 (P <0 . 0 5 ) ;训练 +力竭运动组大鼠外周血淋巴细胞凋亡率较安静组有升高趋势 ,但较力竭运动组有下降趋势 ,脾细胞凋亡率与力竭运动组相近 ,胸腺细胞凋亡率显著高于安静组和力竭运动组 (P <0 . 0 5 ) ;训练 +力竭运动组脾细胞、外周血淋巴细胞内Ca2 +浓度及血清皮质醇浓度显著高于安静组 (P <0 . 0 5 ) ;力竭运动组血清皮质醇浓度亦显著高于安静组。结论 :耐力训练对力竭运动所诱导的血淋巴细胞凋亡有一定的抑制作用 ,对脾细胞凋亡无明显影响 ,还有增加胸腺细胞凋亡率的作用。耐力训练对力竭运动所诱导的血淋巴细胞及脾细胞凋亡的影响可能与阻断了细胞内Ca2 +浓度及血清C水平升高所介导的细胞凋亡信号转导有关。
Objective: To study the effects of endurance training on apoptosis of lymphocytes after exhaustive exercise in rats and to analyze its mechanism. Methods: SD rats were randomly divided into quiet group, exhaustive exercise group, training + exhausted exercise group. After 3-week swimming endurance training, all rats in the training + exhaustive exercise group were subjected to exhaustive swimming with a 4% weight load, and the apoptotic rates of spleen cells, thymocytes and peripheral blood lymphocytes in rats were compared immediately after exercise. Intracellular Ca2 + concentration and serum cortisol (C) concentration. Results: Exhausted swimming time of training + exhaustive exercise group was significantly longer than that of exhaustive exercise group (P <0.05). The apoptosis rate of peripheral blood lymphocytes in training + exhaustive exercise group was higher than that of resting exercise group (P <0.05). The rate of apoptosis of spleen cells was similar to that of exhausted exercise group, and the apoptosis rate of thymus cells was significantly higher than that of quiet group and exhausted exercise group (P <0.05). Training + Exhaustive exercise group spleen cells, peripheral blood lymphocytes Ca2 + concentration and serum cortisol concentration was significantly higher than the quiet group (P <0. 05); exhausted exercise group serum cortisol concentration was significantly higher than the quiet group. Conclusion: Endurance training can inhibit the apoptosis of blood lymphocytes induced by exhaustive exercise, have no obvious effect on the apoptosis of splenocytes, and increase the apoptosis rate of thymocytes. Endurance training on exhaustive exercise-induced apoptosis of blood lymphocytes and spleen cells may be related to the inhibition of intracellular Ca2 + concentration and elevated serum C-mediated apoptosis signal transduction.