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目的 探讨扇贝裙边提取物（ESS）抗动脉粥样硬化（AS）作用,并研究其对血管平滑肌细胞（VSMCs）增殖的影响。方法 1.通过喂饲高脂饲料建立鹌鹑AS模型,观察ESS对血脂水平、组织中脂质含量及动脉粥样硬化斑块形成的影响。2.采用20％胎牛血清（FBS）、碱性成纤维细胞生长因子（bFGF）所诱导的大鼠VSMCs增殖模型,通过细胞计数、结晶紫染色及MTT比色法研究ESS抑制VSMCs增殖作用,并运用免疫组织化学技术（LSAB法）观察ESS对增殖VSMCs的增殖细胞核抗原（PCNA）和血小板衍化生长因子（PDGF）表达的影响。结果 1.与模型组比较,ESS组动物主动脉、冠状动脉内膜AS斑块形成程度明显减轻（P<0.05、P<0.01）,血清总胆固醇（TC）、甘油三酯TC、TG含量均显著降低（P<0.05、P<0.01）。2.ESS各剂量组VSMCs数目及吸光度均明显少于模型组（P<0.05、P<0.01）,并能逆转VSMCs增殖时PCNA、PDGF的表达增强（P<0.01）。结论 ESS具有明显调血脂、抗AS作用,并能显著抑制VSMCs增殖。提示ESS的抗AS作用可能与其调血脂、抑制VSMCs增殖有关。
Objective To investigate the anti-atherosclerotic (AS) effect of scallop skirt extract (ESS) and its effect on the proliferation of vascular smooth muscle cells (VSMCs). Methods 1. Establish a fistula AS model by feeding high-fat diets to observe the effect of ESS on blood lipid levels, lipid content in tissues, and atherosclerotic plaque formation. 2. VSMCs proliferation model induced by 20% fetal bovine serum (FBS) and basic fibroblast growth factor (bFGF) was used to study the effect of ESS on the proliferation of VSMCs by cell counting, crystal violet staining and MTT colorimetry. The effect of ESS on the expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor (PDGF) in proliferating VSMCs was observed by immunohistochemical technique (LSAB). Results 1.Compared with the model group, the formation of plaques in the aorta and coronary endometrial AS of the ESS group was significantly reduced (P<0.05, P<0.01). The serum total cholesterol (TC), triglyceride TC, and TG contents were all significantly reduced. Significantly decreased (P<0.05, P<0.01). 2. The number and absorbance of VSMCs in each dose group of ESS were significantly lower than those in the model group (P<0.05, P<0.01), and the expression of PCNA and PDGF increased when VSMCs were proliferated (P<0.01). Conclusion ESS can significantly regulate blood lipid and anti-AS, and can significantly inhibit the proliferation of VSMCs. It is suggested that the anti-AS effect of ESS may be related to blood lipid regulation and inhibition of VSMCs proliferation.