论文部分内容阅读
目的:探讨白藜芦醇拮抗雷公藤甲素诱导卵巢损伤的作用。方法:40只雌性NIH小鼠随机分为4组:对照组(Ctrl组:含5%二甲基亚砜的生理盐水),模型组(TL组:雷公藤甲素25μg/kg),低剂量治疗组(TL+ResL组:25μg/kg雷公藤甲素+5 mg/kg白藜芦醇)和高剂量治疗组(TL+ResH组:25μg/kg雷公藤甲素+10 mg/kg白藜芦醇)。各组连续灌胃50天。第41天起,行连续阴道涂片。第47天,行超排卵实验。于第50天处死各组小鼠,收集计数输卵管中卵子,留取卵巢行常规病理染色等。阴道涂片行巴氏染色检测小鼠性动周期,HE染色检测小鼠卵巢病理,计数各级卵泡。结果:雷公藤甲素刺激后,TL组小鼠出现明显的卵巢损伤,表现为性动周期延长,超排卵子数量减少,与Ctrl组比较,差异有显著性意义(P<0.05);TL+ResL组和TL+ResH组较TL组小鼠卵巢原始卵泡、初级卵泡、次级卵泡和成熟卵泡数量增多,差异均有显著性意义(P<0.05),TL+ResL组和TL+ResH组比较无差异。结论:白藜芦醇可部分拮抗雷公藤甲素诱导的卵巢损伤,但与白藜芦醇治疗剂量无明显关系。
Objective: To investigate the effect of resveratrol on triptolide-induced ovarian injury. Methods: Forty female NIH mice were randomly divided into 4 groups: control group (Ctrl group: 5% DMSO), model group (TL group: 25 μg / kg triptolide), low dose Treated group (TL + ResL group: 25 μg / kg triptolide + 5 mg / kg resveratrol) and high dose treatment group (TL + ResH group: 25 μg / kg triptolide +10 mg / Resorcinol). Each group continued gavage for 50 days. The first 41 days, the line of continuous vaginal smear. Day 47, superovulation experiments. On the 50th day, the mice in each group were sacrificed and the ovum in the fallopian tube was collected for routine histopathological staining. Vaginal smears were detected by Papanicolaou in mice sexual activity cycle, HE staining was used to detect ovarian pathology in mice, count follicles at all levels. Results: Triptolide induced obvious ovarian damage in TL group, which showed that sexual activity was prolonged and the number of superovulation was decreased. Compared with control group, the difference was significant (P <0.05); TL + Compared with TL group, the number of primary follicle, primary follicle, secondary follicle and mature follicle increased in ResL group and TL + ResH group compared with TL group (P <0.05), TL + ResL group and TL + ResH group No difference. CONCLUSION: Resveratrol partially antagonizes triptolide-induced ovarian injury but has no significant relationship with the dose of resveratrol.