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目的:为观察慢性肝炎及肝硬化患者血浆一氧化氮(NO)与内皮素1(ET-1)和丙氨酸转氨酶(ALT)的水平,并分析其相关性。方法:取慢性乙型肝炎及活动性肝硬化患者血浆,用镉柱法还原NO_3~-,重氮法测NO_2~-,计算血浆中原有及NO_3~-还原后NO_2~-总和(NO_2~-/NO_3~-),放免法测ET-1和赖氏法测定ALT。结果:慢性肝炎轻度(原慢迁肝)、慢性肝炎中度(原慢活肝)及活活动性肝硬化患者血浆NO_2~-/NO_3~-分别为21.92±11.65 μmol/L、33.16+11.89 μmol/L和58.45±27.34 μmol/L。ET-1分别为:56.85±23.43 ng/L、98.45±44.67 ng/L和109.83±49.28 ng/L,ALT各自为87.60±32.50 u、216.50±98.50u、228.40±156.20 iu。与正常对照组比较,慢肝中度及活动性肝硬化患者NO_2~-/NO_3~-及ET-1水平升高较显著(P<0.05-0.01),且呈正相关,慢肝中度与活动性肝硬化患者NO与ALT水平亦呈正相关。结论:慢性乙型肝炎及活动性肝硬化患者内源性NO水平随病情进展而升高,并与ET和ALT水平有一定的相关性。
Objective: To observe the levels of plasma nitric oxide (NO), endothelin-1 (ET-1) and alanine aminotransferase (ALT) in patients with chronic hepatitis and cirrhosis, and analyze their correlation. Methods: The plasma of patients with chronic hepatitis B and active liver cirrhosis was collected. NO_ (3 ~) and NO_ (2 ~ / NO_3 ~ -), radioimmunoassay ET-1 and Lai’s method for the determination of ALT. Results: The levels of plasma NO 2 - / NO 3 - in patients with mild chronic hepatitis (chronic slow-moving liver), moderate chronic chronic hepatitis (chronic active hepatitis) and patients with active cirrhosis were 21.92 ± 11.65 μmol / L and 33.16 ± 11.89 μmol / L and 58.45 ± 27.34 μmol / L. ET-1 were 56.85 ± 23.43 ng / L, 98.45 ± 44.67 ng / L and 109.83 ± 49.28 ng / L, respectively. ALT were 87.60 ± 32.50 u, 216.50 ± 98.50 u and 228.40 ± 156.20 iu, respectively. Compared with the normal control group, the levels of NO 2 - / NO 3 - and ET-1 in chronic moderate and active cirrhosis patients were significantly increased (P <0.05-0.01), and were positively correlated with moderate chronic liver disease activity There is a positive correlation between NO and ALT level in patients with cirrhosis. Conclusion: The levels of endogenous NO in patients with chronic hepatitis B and active cirrhosis increase with the progress of the disease, and have some correlation with ET and ALT levels.