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本文用缩窄腹主动脉和左肾动脉方法复制慢性心功能不全大鼠模型。用放射配体结合实验观察心脏α＿１肾上腺素受体（α＿１－ＡＲ）及其亚型分布的变化，显示在心功能不全大鼠心肌组织上α＿１－ＡＲ的数量及对￣１２５Ｉ－ＢＥ的亲和性与假手术大鼠无显著差别，但α＿１Ａ亚型占α＿１－ＡＲ总数的比例比假手术大鼠明显增高（５１±７％比２２±５％，Ｐ＜０．０１）。离体左心房收缩功能实验结果显示，两组间心肌最大收缩效应无显著差别；当用１０μｍｏｌ／Ｌ酚妥拉明阻断α＿１－ＡＲ时，心功能不全大鼠去甲肾上腺素（ＮＥ）的浓度－收缩效应曲线无显著左移，用１μｍｏｌ／Ｌ苯肾上腺素激动α＿１－ＡＲ时，亦未使异丙肾上腺素浓度－收缩效应曲线右移，用１ｎｍｏｌ／ＬＷＢ４１０１阻断α＿１Ａ亚型时，ＮＥ浓度－收效应曲线不再出现左移，但２０μｍｏｌ／ＬＣＥＣ不可逆阻断α＿１Ｂ亚型后，ＮＥ浓度－收缩效应曲线仍显著右移。结果提示，心功能不全α＿１－ＡＲ及α＿１Ａ亚型对β－ＡＲ介导的正性变力效应的抑制作用消失，而α＿１Ｂ亚型对β－ＡＲ介导的正性变力效应的增强作用仍存在。
In this paper, narrowing the abdominal aorta and left renal artery replication of chronic heart failure rat model. Radioligand binding assay was used to observe the changes of cardiac α 1 -adrenergic receptor (α 1-AR) and its subtypes, showing the number of α 1-AR and the affinity to 125 I-BE in myocardial tissue of rats with cardiac dysfunction Compared with the sham-operated rats, the proportion of α_1A subtype in the total number of α_1-AR was significantly higher than that in the sham-operated rats (51 ± 7% vs. 22 ± 5%, P <0.01). Isolated left atrial systolic function test results showed that the maximum systolic effect between the two groups was no significant difference; when 10μmol / L phentolamine block α_1-AR, cardiac dysfunction in rats norepinephrine (NE) The concentration-contraction effect curve showed no significant shift to the left. When 1μmol / L phenylephrine was used to activate α_1-AR, the isoproterenol concentration-contraction effect curve was not shifted to the right. When α1A subtype was blocked with 1nmol / L WB4101, NE Concentration - income effect curve no longer appear to the left, but 20μmol / LCEC irreversible block α_1B subtype, NE concentration - contraction effect curve is still significantly shifted to the right. The results suggest that the inhibitory effect of α_1-AR and α_1A subtypes on β-AR-mediated positive force disappears, whereas the effect of α_1B subtype on β-AR-mediated positive force exist.