论文部分内容阅读
小鼠骨髓细胞在体外培养条件下所形成的贴壁细胞层,作为模拟的细胞微环境,能够支持多向造血干细胞CFU-S和粒系祖细胞CFU-GM增殖分化达2～3个月。上皮样细胞、成纤维细胞、单核巨噬细胞、脂肪细胞以及细胞之间交错连接所形成的网状支架是贴壁层的基本结构。糖皮质激素氢化可的松对于改善贴壁层的结构,促进造血干细胞的自我更新和向粒系方向分化起重要作用,而受照射的贴壁层则不能维持造血细胞体外长期生长。
The adherent cell layer formed by mouse bone marrow cells under in vitro culture conditions can support proliferation and differentiation of multidrug-derived hematopoietic stem cells CFU-S and granulocyte-derived progenitor cells CFU-GM for 2 to 3 months as simulated cell microenvironment. Reticular scaffolds formed by the interlacing of epithelioid cells, fibroblasts, monocyte-macrophages, adipocytes, and cells are the basic structures of the adherent layer. Glucocorticoid hydrocortisone plays an important role in improving the structure of the adherent layer, promoting the self-renewal of hematopoietic stem cells and the differentiation to the granulocyte, while the irradiated adherent layer can not maintain the long-term growth of hematopoietic cells in vitro.