Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP~(?)4Dx~(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.4.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance. Objective: To evaluate the Anaplasma phagocytophilum (A.phagocytophilum), Ehrlichia canis (E.canis, Dirofilaria immitis (D.immitis) (canine heartworm), Borrelia burgdorferi (B.burgdorferi) infections in rural dogs from Yunnan, Hainan and Anhui provinces . Methods: Serum samples were collected from 26 dogs in Yunnan. Hainan and Anhui province. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP® 4Dx® (IDEXX Laboratories, Inc. USA)). Meaiiwliile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared. Results: The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs positive for E.canu.D.immitis (canine heartworm, and B. burgdorferi by ELISA rapid diagnostic method. number of serologically positive dogs for IgG to A.phagocytophilum was 13 (50%) by IFA method Data of the two methods were analyze d statistical data and the difference was statistically significant (P = 0.002). Conclusions: It can be approved that IFA method was more sensitive than ELISA rapid diagnostic method. Host, we need conduct more and intensive epidemiology survey on tick-born disease pathogens including 4. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.