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目的:探讨Orexins对小鼠摄食和能量代谢的影响。方法:将小鼠分为摄食组和代谢组,摄食组通过中枢置管,注射不同剂量(1、3、10 nmol)的orexin-A和orexin-B,观察它们对小鼠摄食以及肝柠檬酸合酶活性的影响。代谢组将小鼠置于代谢笼内,通过中枢注射orexin-A,观察小鼠在光照条件、黑暗条件、禁食条件下呼吸商和代谢率的变化。结果:与对照组相比,1 nmol和10 nmol orexin-A在注射后4小时内可显著刺激小鼠进食(P<0.05),而3 nmol orexin-A对摄食量的影响并不明显,但能显著促进柠檬酸合酶活性。任何剂量的orexin-B对小鼠摄食都没有显示出刺激作用(P>0.05)。在光照条件下,orexin-A可显著降低呼吸商(RQ),代谢率显著升高(P<0.05);而在黑暗条件下,orexin-A对RQ没有任何影响,但代谢率显著升高(P<0.05);但是给禁食小鼠中注射orexin-A可诱导RQ的短暂升高,代谢率显著升高(P<0.05)。结论:Orexins对小鼠摄食与能量代谢可能有一定的调控作用。
Objective: To investigate the effects of Orexins on ingestion and energy metabolism in mice. Methods: The mice were divided into ingestion group and metabolization group. The feeding group was injected with different dose (1, 3, and 10 nmol) of orexin-A and orexin-B through the central nervous system to observe their ingestion of mice and liver citric acid Synthase activity. Metabolism group mice were placed in metabolic cages, through the central injection of orexin-A, mice under light, dark conditions, fasting conditions, respiratory quotient and metabolic rate changes. Results: Compared with the control group, 1 nmol and 10 nmol of orexin-A significantly stimulated the mice to eat within 4 hours after injection (P <0.05), while 3 nmol of orexin-A had no significant effect on the food intake Can significantly promote citrate synthase activity. No dose of orexin-B showed any irritation to mice (P> 0.05). In light conditions, orexin-A significantly decreased RQ and significantly increased metabolic rate (P <0.05). In the dark, orexin-A had no effect on RQ, but metabolic rate was significantly increased P <0.05). However, administration of orexin-A to fasted mice induced a transient increase in RQ with a significant increase in metabolic rate (P <0.05). Conclusion: Orexins may play a regulatory role in feeding and energy metabolism in mice.