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目的研究浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达的意义。方法利用免疫组织化学链霉亲和素生物素法(labelled streptavidin-biotin method,LSAB)方法检测61例浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)、71例浸润性导管癌(invasive ductal carcinoma,IDC)和27例髓样癌(medullary carcinoma,MC)3种乳腺癌中浸润T淋巴细胞亚群分布以及Th细胞因子的表达。T淋巴细胞组间比较采用Kruskal-WallisTest,组内比较采用Wilcoxon Signed RanksTest;组间Th细胞因子的比较采用χ2检验。结果CD3、CD8和CD4均表达于淋巴细胞胞膜。CD3+T细胞在3组间差异无统计学意义(P=0.735)。CD8+T和CD4+T细胞在3组间差异均有统计学意义(P=0.000,P=0.012)。Th细胞因子主要表达于肿瘤细胞和淋巴细胞的胞质。IL-2表达在IMPC组最低(25/61,41.0%),其次是IDC组(33/71,46.5%),最高是MC组(20/27,74.1%),3组间差异有统计学意义(P=0.014);IFN-γ的表达类似于IL-2,IMPC组最低(39/61,63.9%),其次是IDC组(57/71,80.3%),最高是MC组(24/27,88.9%),3组间差异有统计学意义(P=0.019);IL-4的表达在IDC组最高(42/71,59.2%),其次是IMPC组(19/61,31.1%),最低是MC组(5/27,18.5%),3组间差异有统计学意义(P=0.000);IL-10的阳性率在IMPC组是87.8%(47/61),在IDC组是87.3%(62/71),在MC组是88.9%(24/27),3组间差异无统计学意义(P>0.050)。结论浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达不同。
Objective To study the significance of infiltrating T lymphocytes and Th cytokines in different types of breast cancer. Methods Sixty-one cases of invasive micropapillary carcinoma (IMPC) and 71 invasive ductal carcinoma (IHC) were detected by the method of immunohistochemistry streptavidin-biotin method (LSAB) , IDC) and 27 cases of medullary carcinoma (MC). The distribution of T lymphocyte subsets and the expression of Th cytokines were also observed. Kruskal-WallisTest was used to compare the T lymphocyte groups, and Wilcoxon Signed Ranks Test was used in the group. The comparison of Th cytokines among groups was performed using χ2 test. Results CD3, CD8 and CD4 were all expressed in the lymphocyte cell membrane. CD3 + T cells in the three groups showed no significant difference (P = 0.735). CD8 + T and CD4 + T cells in the three groups were statistically significant (P = 0.000, P = 0.012). Th cytokines are mainly expressed in the cytoplasm of tumor cells and lymphocytes. The expression of IL-2 was the lowest in IMPC group (25 / 61,41.0%), followed by IDC group (33/71, 46.5%) and the highest in MC group (20/27, 74.1%). The difference among the three groups was statistically (39/61, 63.9%) in the IMPC group, followed by the IDC group (57/71 and 80.3%), the highest in the MC group (24 / (P = 0.019). The expression of IL-4 in the IDC group was the highest (42/71, 59.2%), followed by the IMPC group (19/61, 31.1%), the difference was statistically significant , And the lowest was MC group (5/27, 18.5%). The difference between the three groups was statistically significant (P = 0.000). The positive rate of IL-10 was 87.8% (47/61) in IMPC group, 87.3% (62/71) in the MC group, and 88.9% (24/27) in the MC group. There was no significant difference among the three groups (P> 0.050). Conclusion The expressions of infiltrating T lymphocytes and Th cytokines in different types of breast cancer are different.