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[目的]优化欧美杨108的无性繁殖再生体系的培养条件。[方法]以欧美杨108无菌苗为材料,采用正交试验优化叶片直接分化和茎段愈伤组织分化再生体系的培养条件。[结果]欧美杨108的叶片适宜在光照下进行直接再生分化,茎段适宜在光照下进行愈伤组织诱导再生分化。叶片不定芽分化的培养基为MS基本培养基(琼脂7.0 g/L、pH 6.0、蔗糖20 g/L)附加0.6 mg/L 6-BA和0.2 mg/L NAA;茎段愈伤组织诱导培养基为WPM固体培养基附加0.75 mg/L KT和1.5 mg/L 2,4-D。不定芽诱导生根培养基为WPM固体培养(蔗糖30 g/L)附加2.0 mg/L IBA。[结论]该研究优化了欧美杨108叶片的直接分化再生体系和茎段愈伤组织再生体系的培养条件,为其组织培养及遗传转化体系的构建提供了基础支持。
[Objective] The research aimed to optimize the culture conditions of clonal regeneration system of Populus × euramericana 108. [Method] With the sterile culture seedlings of Populus × euramerican 108 as material, the orthogonal experiment was used to optimize the culture conditions of direct leaf differentiation and regenerative regeneration of stem callus. [Result] The leaves of Populus × euramericana 108 were suitable for direct regeneration and differentiation under light irradiation. The stem segments were suitable for callus induction and regeneration differentiation in light. Adventitious bud differentiation of leaves was supplemented with 0.6 mg / L 6-BA and 0.2 mg / L NAA in MS medium (agar 7.0 g / L, pH 6.0, sucrose 20 g / L) Additional 0.75 mg / L KT and 1.5 mg / L 2,4-D were added to WPM solid media. Adventitious buds were induced by rooting medium supplemented with 2.0 mg / L IBA in WPM solid culture (sucrose 30 g / L). [Conclusion] The research optimized the culture conditions of the direct differentiation regeneration system and regeneration system of stem callus of Populus × euramericana 108 leaves, which provided basic support for the tissue culture and genetic transformation system construction.