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目的用不同的溶剂在常温下对橘皮进行提取,可以得到不同的橘皮提取物。方法以下分析了石油醚、氯仿、乙酸乙酯、正丁醇、去离子水5种不同的橘皮提取物对1,1-二苯基-2-苦基肼(DPPH)与亚硝酸盐的清除能力,并进一步的分析橘皮提取物中的有效成分及其含量。结果不同溶剂的橘皮提取物都对DPPH与亚硝酸盐有清除能力,只是清除能力的强弱有一定的差别,其中的乙酸乙酯的清除能力表现的最强。结论乙酸乙酯橘皮提取物中的各成分含量最高,通过对5种提取物的性能进行对比分析,橘皮提取物中的黄酮含量是影响清除DPPH与亚硝酸盐的能力主要相关因素。
Objective To extract orange peel with different solvents at room temperature to obtain different orange peel extracts. Methods The effects of petroleum ether, chloroform, ethyl acetate, n-butanol and deionized water extracts on the biodegradation of 1,1-diphenyl-2-picrylhydrazine (DPPH) and nitrite Cleansing ability, and further analysis of the active ingredient in the orange peel extract and its content. Results Different extracts of orange peel had scavenging ability to DPPH and nitrite, but there was a certain difference in the abilities of scavenging ability, of which ethyl acetate scavenging ability was the strongest. Conclusion The content of the components in the ethyl acetate orange peel extract is the highest. By comparing the performance of the five extracts, the content of flavonoids in the orange peel extract is the main factor that affects the ability of scavenging DPPH and nitrite.