依据宇佐美曲霉(Aspergillus usamii)E001木聚糖酶(Xyn Ⅱ)N末端氨基酸残基序列以及曲霉菌密码子的偏爱性和真核生物mRNA在3'......

以宇佐美曲霉（Aspergillus usamii）E001株基因组DNA为模板，PCR扩增了编码木聚糖酶Ⅱ（Xylanases，XynⅡ）成熟肽的DNA片段。构建重组克隆质......

采用缓冲液浸提、硫酸铵盐析、Phenyl- Sepharose CL- 4B疏水层析和Sephadex G- 75凝胶过滤层析等分离纯化手段,从宇佐美曲霉E001......