streptavidin相关论文
分子动力学模拟可以用来研究生物大分子体系的各种性质,如：预测受体和配体之间的亲和力,模拟蛋白质的折叠过程,研究蛋白质与细胞膜......
The specific binding of receptor to ligand covalently attached to surface with different surface densities was studied u......
Combinational biosynthesis of novel dual-functional phycobiliproteins and its application in early d
The development of fluorescent tools with high fluorescence quality and efficient targeting is of great importance in im......
Salt bridge exchange binding mechanism between streptavidin and its DNA aptamer-Thermodynamics and s
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Development of a test strip based on DNA-functionalized gold nanoparticles for rapid detection of me
A rapid,sensitive,selective and reliable strip assay based on DNA-functionalized gold nanoparticles for Hg~(2+) detectio......
The specific binding of receptor to ligand covalently attached to surface with different surface densities was studied u......
It is extremely important for bacteria detection in many fields,such as medical diagnosis and food safety.In this paper,......
目的发展一种测定基因转殖酵母菌培养液中生物素的灵敏ELISA竞争测定法.方法酶标板先用猪肺炎支原体包被,再依次用兔抗猪肺炎支原......
用LSAB（Labelled streptavidin biotin method）免疫组化法对30例白血病骨髓细胞进行了P_（21）蛋白免疫组化检测。其中急性粒细胞性白血......
AIM:To study the relationship between infection withHelicobacter pylori L-forms and chronic gastritis and itsassociatio......
Relationship between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its
Objective To explore the role and significance of matrix metalloproteinase-9(MMP-9)in angiogenesis through observing the......
AIM: To explore the effects of liniment levamisole on cellular immune functions of patients with chronic hepatitis B. ME......
作者介绍Ｓｔｒｅｐｔａｖｉｄｉｎ－ｇｏｌｄ探针的制备、纯化、鉴定及应用情况．以该探针所做胃癌组织中凝集素受体及胃癌单抗相应抗原的光镜与电镜定位，均取得满意结果......
Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute Tlymphocyte leukemia cell ......
A sensitive and specific ELISA for human thyrotropin(hTSH) is established with using two anti-hTSH monoclonal antibody. ......
Objective:To labelavidin(Av)or streptavidin(SA)with 153 Sm by takingadvantageof thehighbindingaffin-ityof biotinto......
Functionalized liposomes were prepared by mixing the biotin in the lipid vesicle suspensions. The experiments through ......
Objective:To investigate the expressions and their relationship of pituitary tumor transforming gene(PTTG)and proliferat......
[目的]探讨中波紫外线(UVB)对正常小鼠表皮细胞凋亡及凋亡相关基因Bax和Bcl-2的调控。[方法]将正常ICR小鼠随机分为对照组(假照射......
据英《新科学家》2003年4月19日报道 :一种能够可靠地检测和分析各种蛋白质的微型传感器 ,已分别由美国的两个科研小组研制出来。......
,Resolution improvement of electron micrographs by the direct method for biotin-binding protein stre
Direct-method phase extension has been applied to two-dimensional electron diffraction data of the protein streptavidin.......
In this study, we developed a systematic evolution of ligands by exponential enrichment (SELEX) method using a combinati......
目的 建立测定乙酰肝素酶(HPA)的生物素-链亲和素系统(BSA) TRFIA并进行初步应用.方法 采用部分基因重组的鼠抗人HPA单克隆抗体包......
Background and Objective: Intravesical administration of Bacillus Calmette-Guerin (BCG) after transurethral resection is......
目的 探讨链亲和素标记的白细胞介素4( IL-4-SA)双功能融合蛋白治疗小鼠浅表性膀胱癌的效果.方法 制备IL-4-SA双功能融合蛋白,测定......
Two kinds of streptavidin magnetic particles, namely streptavidin GoldMag particles and streptavidin amino terminal part......
以生物素标记的ＥＢ病毒Ｗ片段为探针，亲和素为检测手段，探索了在常规石蜡切片标本中检测ＥＢ病毒ＤＮＡ的原位分子杂交技术。通过简化标本杂交前的处......
SP方法全称为链菌素亲生物素——过氧化酶连接法(Streptavidin Peroxidase Con-jugated Method).该方法首先由美国Zymed公司于1986......
1990年8月上旬,日本防卫医大病理科医学博士河合俊明副教授来我院讲学,并为我们指导实习了标记式链霉卵白素生物素(labelled strep......
目的 建立一种简便，快速，准确检测人心肌肌钙蛋白Ｔ（ｃＴｎＴ）的胶体金免疫层析法（ＧＩＣＡ）。方法 制备的胶体金标记抗ｃＴｎＴ单克隆抗体３Ｆ７，生物素标记另一株抗体２Ｈ８，链......
应用链霉亲和素和生物素系统发展成的酶连接免疫吸附测定（ELISA）改良法检测HBeAg，取得较满意效果。标记链霉亲和素-辣根过氧化物酶（SA-......
为了降低鸟亲和素的高肝摄取，延长Ａｖ在血液中的滞留时间，对Ａｖ进行化学修饰，修饰后的Ａｖ衍生物用＾１２５Ｉ标记示踪，观察小鼠体内生物分布。结果表明，Ａｖ衍生......
Aim To develop a sensitive competitive ELISA for the determination of biotin in transformed yeast culture media.Methods ......
【目的】利用细胞融合技术及细胞免疫组化方法,筛选可特异性阻断鸡传染性法氏囊病病毒（IBDV）感染的抗鸡胚成纤维细胞（CEF）膜蛋白单克隆......
制备凝血靶向通用效应因子tTF／SA融合蛋白，并鉴定其生物学活性。利用PCR技术构建tTF与链霉亲和素SA的融合基因，克隆至表达裁体pET22b（＋），......
本文报道链霉亲和素(streptavidin.SA)的产生、纯化和主要特性鉴定。根据Streptomycesavidinii菌对碳、氮源的需求,配制成特定培养......
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提出了一种将光纤传感技术与生物反应放大技术相结合的方案，实现了链酶亲和素浓度的检测。该方案采用硅烷偶联的方法对光纤进行表面......
采用改进的Polyol合成法，以PEO-PPO-PEO为表面活性剂制备了链霉亲和素-异硫氰酸荧光素偶联的Fe3O4／Au纳米粒子；利用透射电镜和X射线衍......
建立时间分辨荧光核酸杂交分析体系应用于临床乙型肝炎病毒ＤＮＡ检测。采用本室自制螯合剂异硫氰酸苯基－ＥＤＴＡ制备铕离子标记的链霉亲和素，抽提......
目的对链霉亲合素（streptavidin，SA）预包被酶联反应板的优化方法进行研究，比较干法、湿法包被的优越性。方法通过对包被缓冲液、SA浓度......
目的：探讨磁共振（MR）预定位免疫成像中SA—DTPA—Gd对靶瘤性的致增强作用。方法：两组荷人结肠癌细胞裸鼠分别经静脉注射SA—DTPA—Gd和......
主要阐述了流式细胞仪在无脊椎动物血细胞分类、细胞免疫学、定量流式细胞分析、染色体核型分析、细胞核、细胞受体分析等研究中的......
目的：制备链亲合素标记的TNFα双功能融合蛋白,并对其活性进行研究。方法：构建原核表达质粒pET24a-SA-TNFα和pET21a-TNFα-SA;转化......
目的：转铁蛋白受体特异性富含于血脑屏障和肿瘤细胞的表面,是当前中枢神经系统疾病和肿瘤治疗中定向转运的重要靶标.拟获得在大肠杆......
目的制备链亲和素标记的鼠白介素4（mIL-4-SA）融合蛋白,并研究其生物学功能。方法通过RT-PCR克隆mIL-4基因,构建mIL-4-SA-pET21表达质......
A novel immunotherapy for superficial bladder cancer by the immobilization of streptavidin-tagged bi
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金纳米粒子二聚体在纳米科学技术的诸多领域中展示出引人注目的成就，但金纳米粒子二聚体的制备仍然是一项具有挑战性的工作。文章对......
目的探讨利用生物素-亲和素系统的靶向定位效应和生物放大作用,提高MR分子成像的敏感性.方法制备生物素化HAb18单克隆抗体并测定其......
目的 研究比较链霉亲合素（SA）-DTPA-Gd经腹腔和静脉注射后对荷瘤裸鼠体内肿瘤的强化效应，以提高磁共振（MR）靶向成像的敏感性。方法 12只......
目的制备链亲和素（SA）／人白细胞介素-15（hIL-15）融合蛋白SA—hIL-15和hIL-15-SA，并鉴定其生物学活性。方法构建原核表达质粒pET24a-6His-......
