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In the present study, we describe the isolation heterotrophic nitrifier with high ammonium and nitrite nitrogen removal ability from the aquaculture pond, and the nitrification performance of mixed culture of multi-strains.The strains were isolated from sediment, waters and intestinal tract of fish samples in the high density aquaculture ponds by enriching with NH4C1 and NaNO2 as a sole nitrogen source respectively and were identified by sequence analysis of 16S rRNA.Ammonium and nitrite nitrogen removal ability were investigated under aerobic conditions.