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Background:Because of minute anatomic structures,weak sound signals,less studies and paradoxical results,there is still a great deal of debate concerning whether mice cough in response to tussive stimuli.Indian and Mexican scholars placed the mice which had been exposed to stimuli in an up-ended filter funel with a stethoscope at the tip and observed the frequency of mice cough.But mice cough sound could not be recorded,therefore the results were subjective.Junzo Kamei insisted on mouse cough experiments for decades.Mice were half-stationarily exposed to a nebulized stimuli under conscious and identical conditions.