【摘 要】
：
Trisporic acid(TA),a well-known sex hormone of the Mucorales,stimulated the production of β3-carotene[1,2].In this study,the performance of TA on four macroporous resins (AB-8,HP2MGL,SP207 and SP850)
【机 构】
：
State laboratory of chemical resource engineering,Beijing University of Chemical Technology,Beijing,
【出 处】
：
The 6th International Conference on Separation and Technolog
论文部分内容阅读
Trisporic acid(TA),a well-known sex hormone of the Mucorales,stimulated the production of β3-carotene[1,2].In this study,the performance of TA on four macroporous resins (AB-8,HP2MGL,SP207 and SP850) was evaluated according to their adsorption and desorption properties.SP850 resin showed the best adsorption and desorption capacity for TA than other resins,and its adsorption data suited well to the Freundlich and Langmuir isotherm [3].To optimize the separation process of TA from the fermentation broth,dynamic adsorption and desorption experiments were conduced with the column packed with SP850 resin.The optimal conditions were:the sample solution pH 2,flow rate 6BV/h,temperature 25℃.The gradient elution program was as follows:elution solvent ethanol-water(30:70,v/v) 3 BV then ethanol-water(60:40,v/v) 8BV,flow rate 9BV/h.After the SP850 resin treatment,the puiryof TA was 70%.We conclude separation trisporic acid from the fermentation broth using macroporous resins may cause great economic value.
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