Purpose: To evaluate the biological effects of lipopolysaccharide-amine nano-polymersomes/hyaluronic acid polyelectrolyte membrane to bone marrow mesenchymal stem cells on titanium surface.Methods: Cationic polyelectrolyte is lipopolysaccharide-amine nano-polymersomes.Anionic polyelectrolyte is hyaluronic acid.Polyelectrolyte films were constructed by Layer by layer selfassembly technique.The morphology of titanium surface was observed by scanning electron microscopy before and after assembles.Physical and chemical characterization of polyelectrolyte films measured by UV spectroscopy and contact angle measurements.The adhesion,proliferation,differentiation and originally transfection capability of bone marrow mesenchymal stem cells on the polyelectrolyte films were studied as well.