南海海洋生物共附生微生物及其代谢产物的多样性研究

来源 :第五届岭南有机化学论坛暨华南理工大学-兰州大学有机化学双边论坛 | 被引量 : 0次 | 上传用户:haoliangli
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我国南海有丰富的珊瑚、海星,海绵等物种,这些海洋生物的体表和体内共附生有大量的海洋微生物(包括细菌、真菌、放线菌等).近几年来,我们对采自三亚国家珊瑚礁保护区的一批软珊瑚、海绵、长棘海星等生物样品的共附生真菌,进行了分离纯化,获得近1000 余株纯的真菌菌株.采用通用引物ITS1 和ITS4 扩增其中代表性真菌的ITS-rDNA 序列并进行测序确定其分子地位,利用邻接法构建系统发育树及简单分析真菌种群多样性.
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