Structure,Catalysis,and Inhibition of OfChi-h,the Lepidoptera-exclusive Insect Chitinase

来源 :中国生物化学与分子生物学会第十二届会员代表大会暨2018年全国学术会议 | 被引量 : 0次 | 上传用户:lilinchang0105
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Chitinase-h(Chi-h)is of special interest among insect GH18 chitinases due to its exclusive distribution in lepidopteran insects,one of the most destructive crop pests.Proteomic analysis of insect molting fluid as well as RNAi of SeChi-h from Spodoptera exigua indicating that Chi-h is indispensable for molting.
其他文献
本研究以产微球茎菌属 ALW1(Microbulbifer sp.ALW1)的基因组为模板进行PCR扩增,得到昆布多糖酶Glu3558的基因片段后将其插入到pPIC9K质粒中构成重组质粒.将重组质粒转化到E.coli DH5α感受态细胞中,通过含有氨苄青霉素抗性的平板筛选、菌落PCR和序列鉴定,确认重组质粒构建成功.
The gene of chondroitinase 1816(ChSase1816)was overexpressed and purified from Escherichia coli BL21(DE3)with a molecular mass of 86 kDa.Using the chondroitin sulfate B(CS-B)as the substrate,the Km an
Temperature is a key determinant factor for lifespan in both poikilotherms and homeotherms.It is believed that animals live longer at lower body temperatures.However,the precise role of temperature re
Staphylococcal enterotoxin C2(SEC2)causes rapid clonal expansion of lymphocytes and secretion of T cell growth factors,leading to a severe inflammatory response within tissues.Our previous studies con
Recombinase polymerase amplification(RPA)is a novel nucleic acid amplification technology that does not require repeated control of temperature and precision instruments.It has the characteristics of
As a newly discovered tumor suppressor,PAQR3 is a Multifunctional protein.To identify the new potential functions,we applied a proximity labeling strategy exploiting APEX2-fused PAQR3 to biotinylate p
[Objective]To investigate the effects of Soybean Extracts administration on the protein expression levels of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-l(TIMP-l)in athe
Bleomycin,a widely used cancer drug,may give rise to a serious side effect,pulmonary fibrosis which is associated with significant morbidity and mortality.Despite the intensive efforts,the precise pat
Rheumatoid arthritis(RA)is a common chronic autoimmune inflammatory disorder.The role of autophagy and apoptosis in the pathogenesis of RA is intricate.Interleukin-22(IL-22)as a cytokine of Th22 plays
It is widely demonstrated that aberrantly expressed lncRNAs are associated with various cancers,but it is still uncertain how they may become coordinated during the gastric carcinogenesis.Here,we repo