【摘 要】
:
本研究以产微球茎菌属 ALW1(Microbulbifer sp.ALW1)的基因组为模板进行PCR扩增,得到昆布多糖酶Glu3558的基因片段后将其插入到pPIC9K质粒中构成重组质粒.将重组质粒转化到E.coli DH5α感受态细胞中,通过含有氨苄青霉素抗性的平板筛选、菌落PCR和序列鉴定,确认重组质粒构建成功.
【机 构】
:
集美大学食品与生物工程学院,福建厦门,361021
【出 处】
:
中国生物化学与分子生物学会第十二届会员代表大会暨2018年全国学术会议
论文部分内容阅读
本研究以产微球茎菌属 ALW1(Microbulbifer sp.ALW1)的基因组为模板进行PCR扩增,得到昆布多糖酶Glu3558的基因片段后将其插入到pPIC9K质粒中构成重组质粒.将重组质粒转化到E.coli DH5α感受态细胞中,通过含有氨苄青霉素抗性的平板筛选、菌落PCR和序列鉴定,确认重组质粒构建成功.
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