【摘 要】
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OBJECTIVE To investigate the effect of SBF on cortical cytoplasm apoptotic factors disturbances induced by complex Aβ25-35 in rats.METHODS The cerebral injuries model was establised by rats received i
【机 构】
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Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development/Institute of
【出 处】
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第十六届全国神经精神药理学学术会议
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OBJECTIVE To investigate the effect of SBF on cortical cytoplasm apoptotic factors disturbances induced by complex Aβ25-35 in rats.METHODS The cerebral injuries model was establised by rats received intracere-broventricular injection of RHTGF-β1, Aβ 25-35 and AlCl3 and then accepte SBF trestment.All the rats were sacrificed by decapitation for indicators detection at last the drug treatment.Western blotting method was for cysteinyl aspartate specific protease-3 (caspase-3) protein expression and RT-PCR method detected cytochrome C, apoptotic protease activating factor-1 (Apaf-1), cysteinyl aspartate specific protease-9 (caspase-9) mRNA expression in cortical cytoplasm.RESULTS The protein expression of caspase-3 in cortical cytoplasm of rats by Western blotting.The results indicated that compared with the sham group,the caspase-3 protein expression of cortical cytoplasm in Aβ group was significantly increased (P<0.01).However,the increased expression can be obviously reversed by SBF at does of 35, 70 and 140 mg · kg-1 , as compared with model group (P< 0.01).The Cyt-C, Apaf-1 and caspase-9 mRNA expressions in cortical cytoplasm of rats by RT-PCR.The results indicated that compared with the sham group, the Cyt-C Apaf-1 and caspase-9 mRNA relative expressions of cortical cytoplasm in Aβ group was significantly increased (P<0.01).However, these increased expressions can be differently reversed by SBF 35, 70 and 140 mg·kg-1, as compared with model group (P<0.01).CONCLUSION SBF can definitly improve rats cortical cytoplasm apoptotic factors disorders induced by complex Aβ25-35, which maybe benefit for treatment of degenerative disease.
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