【摘 要】
：
To find a way to promote the rate of carbon flux and further improve the photosynthetic rate in rice,two CO2-transporting and fixing relevant genes,Ictb and FBP/Sbpase,which were derived from cyanobac
【机 构】
：
State Key Laboratory for Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan,China;Engineeri
论文部分内容阅读
To find a way to promote the rate of carbon flux and further improve the photosynthetic rate in rice,two CO2-transporting and fixing relevant genes,Ictb and FBP/Sbpase,which were derived from cyanobacteria with the 35SCaMV promotor in the respective constructs,were transformed into rice.Three homologous transgenic groups with Ictb,FBP/Sbpase and the two genes combined were constructed in parallel,and the functional effects of these two genes were investigated by physiological,biochemical and leaf anatomy analyses.The results indicated that the mesophyll conductance,net photosynthetic rate and carboxylation efficiency were higher at approximately 10.5-36.8%,13.5-34.6%and 21-33.9%,respectively,in the three groups but without any changes in leaf anatomy structure compared with wild type.Other physiological and biochemical parameters increased with the same trend in the three groups,which showed that the effect of FBP/SBPase on improving photosynthetic capacity was better than that of ICTB and that there was an additive effect in ICTB+FBP/SBPase.ICTB localized in the cytoplasm,whereas FBP/SBPase was successfully transported to the chloroplast.The two genes might show a synergistic interaction to promote carbon flow and the assimilation rate as a whole.The multigene transformation engineering and its potential utility for improving the photosynthetic capacity and yield in rice are discussed.
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