Magnaporthe oryzae, the causal agent of rice blast, has been used as a model for investigating the interaction between phytopathogenic fungi and plants.Identification genes involved in mycelial growth of M.oryzae will contribute to reveal their functions in mycelial growth and to establish the foundation for the future construction of the gene network for regulation of the mycelial growth in M.oryzae.MGG_01005 encodes a protein belonging to dynein light chain/Tctex-1 family, and the protein was named as MoTetex.Compared with the wild type, the delta-MoTctex mutants grew slower and formed less conidia, indicating that MoTctex plays vital roles in vegetative growth and conidia formation.The subcellular localization examination demonstrated that the MoTctex-GFP fusion protein was mainly located into cytoplasm of conidia and vegetative hyphae.To further understand the molecular bases of the function of MoTctex in M.oryzae, the authors have expressed the full-length recombinant protein in Escherichia coli.The purified protein was crystallized by vapor diffusion method and the crystals were subsequently used for X-ray diffraction analysis to obtain data at 2 (A)resolution.The crystal structure of the protein will be solved by SAD (Single--wavelength anomalous dispersion) method and the interaction between it and other proteins will be anayzed by GST pull down, yeast two-hybrid and other biophysical methods.