【摘 要】
：
目的:通过测定蒙古口蘑多糖(Mongolia Tricholoma polysaccharide,MOP)的抗氧化活性,观察其在低密度脂蛋白(Low density lipoprotein,LDL)被氧化修饰过程中的影响及其在抗内皮细胞凋亡的作用,探讨其可能的保护血管、抗AS的作用机制.方法:将蒙古口蘑子实体用无水乙醇进行脱脂处理后90℃加热6h进行多糖提取.将得到的粗多糖溶液用3倍体积的无水乙醇
【机 构】
：
内蒙古大学生命科学学院生物系;呼和浩特职业学院生物化学研究所 内蒙古大学生命科学学院生物系
【出 处】
：
2014年第十二届全国脂质与脂蛋白学术会议
论文部分内容阅读
目的:通过测定蒙古口蘑多糖(Mongolia Tricholoma polysaccharide,MOP)的抗氧化活性,观察其在低密度脂蛋白(Low density lipoprotein,LDL)被氧化修饰过程中的影响及其在抗内皮细胞凋亡的作用,探讨其可能的保护血管、抗AS的作用机制.方法:将蒙古口蘑子实体用无水乙醇进行脱脂处理后90℃加热6h进行多糖提取.将得到的粗多糖溶液用3倍体积的无水乙醇,4℃过夜沉淀.用Sevage法(氯仿:正丁醇=4∶1)除蛋白,经醇沉淀,依次用乙醇、丙酮和乙醚洗涤.最后得到较纯的多糖.DPPH法检测MOP的清除自由基活性.将LDL分为MOP处理组和未处理组,经Cu2+氧化处理后,ELISA试剂盒检测它们被氧化程度.同时用这两组被氧化的LDL(ox-LDL)刺激血管内皮细胞,用荧光倒置显微镜和流式细胞仪检测细胞凋亡.
其他文献
Ure2 is the protein determinant of the [URE3] prion state in Saccharomyces cerevisiae,and it forms amyloid fibrils in vitro via conformational change.At the early stages of amyloid fibril assembly,oli
A colorimetric and fluorescent chemosensor bearing hybrid coumarin-hemicyanine functionality has been synthesized for H2S sensing.The detection of sulfide was performed via the nucleophilic attack tow
The detection property of natural drug-curcumin (1) was firstly reported by us.Experimental details showed 1 acts as an excellent UV-Vis and fluorescent selectivity,sensitivity probe for the detection
Using the stopped-flow circular dichroism (SFCD) technique,we investigate the kinetics of pH-induced folding and unfolding processes of DNA i-motif.It is.found that the molecule can fold or unfold at
一些金属蛋白酶在金属离子的作用下可以发生构象的转变,从而产生特定的生物活性位点发挥其生物学功能[1].蛋白-蛋白之间的相互作用可以用于构建新型材料[2],借鉴金属蛋白酶的这种刺激响应性,本研究中设计具有金属离子结合位点的多肽分子,预期多肽分子可以在金属离子的作用下发生构象转变.
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established to quantify sialic acid (N-acetylneuraminic acid, NANA) from human lipoproteins.The method was used to investigate th
Object: There are two isoforms of apolipoprotein B (apoB) in mammals: apoB-100 and apoB-48.The latter is generated by C-to-U editing of apoB mRNA, catalyzed by multiple apoB RNA editing enzyme compone
Background: The advancesin high-throughput technologies have allowed for the massive analysis of genomic data, providing benefits for the characterization of transcriptome architectures.Recent studies
Introduction: Non-alcoholic fatty liver disease (NAFLD), the hepatic feature of the metabolic syndrome, is the most prevalent chronic liver diseases in developed countries and urban population of Chin
Background and purpose: Tanshinone ⅡA is an active component of a traditional Chinese medicine.This study aimed at investigating the effect of tanshinone ⅡA on cholesetrol reverse transport and HDL su