• 不 限
  • 期刊论文
  • 硕博论文
  • 会议论文
  • 报 纸
  • 英文论文
  2. 您的位置
  3. 首页
  4. 会议论文
  5. Quantitative measurement of sialic acid from lipoproteins by liquid chromatography tandem mass spect

Quantitative measurement of sialic acid from lipoproteins by liquid chromatography tandem mass spect

来源 :2014年第十二届全国脂质与脂蛋白学术会议 | 被引量 : 0次 | 上传用户：yangmu2003
【摘 要】
：
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established to quantify sialic acid (N-acetylneuraminic acid, NANA) from human lipoproteins.The method was used to investigate th
【作 者】
：
GUO Shoudong SANG Hui YANG Nana KAN Yujie Li Fuyu LI Yu LI Fangyuan QIN Shucun 
【机 构】
：
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University
【出 处】
：
2014年第十二届全国脂质与脂蛋白学术会议
【发表日期】
：
2014年7期
下载到本地 , 更方便阅读
下载此文 赞助VIP
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established to quantify sialic acid (N-acetylneuraminic acid, NANA) from human lipoproteins.The method was used to investigate the content difference of liproprotein NANA between diabetic (average age 51.6 years) and healthy participants (average age 50.7 years).Sialic acid from lipoprotein samples were released by acetic acid (pH=2) at 80 ℃ for 2 h and analyzed by the optimized LC-MS/MS method after high speed centrifugation and filtration.The limit of detection and quantification of NANA was 7.4 and 24.5 pg, respectively.
其他文献
8-bromo-5-hydroxy-7-methoxychrysin targeted for inhibiting the properties of liver cancer stem cells
Emerging evidence has suggested that cancer stem cell with expression of surface biomarkers including CD 133 and CD44 have more aggressive biological behavior, including epithelial-mesenchymal transit
会议
hepatocellular carcinomacancer stem cell8-bromo-7-methoxychrysintwistepithel
A New Fluorescence Polarization Method for Simultaneous Position and Orientation Measurements of Sin
Simultaneous measurements of single molecule position and orientation are essential for deciphering molecular mechanisms that involve dynamic conformational changes.We developed a new single molecule
会议
Single Molecule Fluorescence PolarizationFluorescence AnisotropyConformational
Super-resolution Imaging and Tracking of Protein-Protein Interactions in Sub-diffraction Cellular Sp
Imaging localization and dynamics of individual interacting protein pairs is essential but often difficult due to the fluorescent background from other paired and non-paired molecules,particularly in
会议
BiFCPALMprotein-protein interactionMreBEF-Tu
Single-molecule and ensemble FRET study of the conformational dynamics of human Hsp70
Hsp70 is a conserved molecular chaperone which plays an indispensable role in regulating protein folding,oligomerization,and degradation in prokaryotic and eukaryotic organisms.Hsp70 consists of an N-
会议
single molecule detectionFRETHsp70molecular chaperoneallostery
Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Ce
Paclitaxel (PTX),a chemotherapeutic drug,affects microtubule dynamics and influences endocytic trafficking.However,the mechanism and the dynamics of altered endocytic trafficking by paclitaxel treatme
会议
Observation of conformational changes during amyloid fibril assembly of Ure2 by single-molecule tech
Ure2 is the protein determinant of the [URE3] prion state in Saccharomyces cerevisiae,and it forms amyloid fibrils in vitro via conformational change.At the early stages of amyloid fibril assembly,oli
会议
single-molecule techniqueamyloid fibrilUre2conformational change
A Ratiometric Colorimetric and Fluorescent Chemosensor for Rapid Detection Hydrogen Sulfide and Its
A colorimetric and fluorescent chemosensor bearing hybrid coumarin-hemicyanine functionality has been synthesized for H2S sensing.The detection of sulfide was performed via the nucleophilic attack tow
会议
RatiometricColorimetricFluorescentHydrogen SulfideBioimaging
The application of natural drug-curcumin in the detection hypochlorous acid of real sample and its b
The detection property of natural drug-curcumin (1) was firstly reported by us.Experimental details showed 1 acts as an excellent UV-Vis and fluorescent selectivity,sensitivity probe for the detection
会议
CurcuminHypochlorous AcidDetectionBioimagingApplication
Study of pH-Induced Folding and Unfolding Kinetics of the DNA i-Motif by Stopped-Flow Circular Dichr
Using the stopped-flow circular dichroism (SFCD) technique,we investigate the kinetics of pH-induced folding and unfolding processes of DNA i-motif.It is.found that the molecule can fold or unfold at
会议
金属离子对多肽分子组装的影响
一些金属蛋白酶在金属离子的作用下可以发生构象的转变,从而产生特定的生物活性位点发挥其生物学功能[1].蛋白-蛋白之间的相互作用可以用于构建新型材料[2],借鉴金属蛋白酶的这种刺激响应性,本研究中设计具有金属离子结合位点的多肽分子,预期多肽分子可以在金属离子的作用下发生构象转变.
会议
多肽金属离子组装