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Berberine is one of active alkaloids derived from Coptidis rhizoma in traditional Chinese medicine.The pharmacokinetics of berberine in rat plasma were compared between normal and chronic visceral hypersensitivity irritable bowel syndrome rats (CVH-IBS).CVH-IBS were established by mechanical colon irritation using angioplasty balloons for two weeks after oral administration of berberine hydrochloride (25 mg/kg) with an equivalent dose of 22 mg/kg of berberine by body weight.An immunohistochemical analysis of c-fos and myosin light chain kinase (MLCK) and immunofluorescence analysis of MLCK in rat colons were conducted.Quantification of berberine in rat plasma was achieved using a sensitive and rapid UPLC-MS/MS method.