【摘 要】
：
Although considerable clinical attempts on various types of cancers have achieved,photodynamic therapy(PDT)still suffers attenuated therapeutic effects due to the developed resistance of cancer cells.
【机 构】
：
College of Chemistry & Environmental Science,Chemical Biology Key Laboratory of Hebei Province,Key L
论文部分内容阅读
Although considerable clinical attempts on various types of cancers have achieved,photodynamic therapy(PDT)still suffers attenuated therapeutic effects due to the developed resistance of cancer cells.As a member of anti-apoptosis protein family,survivin has been demonstrated to be selectively overexpressed in most human malignant tumors and plays a significant role in cancer progression and therapeutic resistance.Herein,we present an upconversion nanoplatform for enhanced PDT by DNAzyme-mediated gene silencing of survivin.In our system,a long single-stranded DNA(ssDNA)with repeated aptamer(AS1411)and DNAzyme was fabricated by rolling circle amplification(RCA)and adsorbed on the upconversion nanoparticles(UCNPs)by the electrostatic attraction.The multivalence of the ssDNA endows the upconversion nanoplatform with high recognition and loading capacity of photosensitizers and DNAzymes.Once the nanoplatform is targeted delivered into cancer cells,the PDT can be activated by near-infrared(NIR)light to generate ROS for killing the cancer cells.And the encoded DNAzyme can efficiently inhibit the expression of survivin gene,allowing the potential to enhance the efficiency of PDT.Our work thus highlights the promise of upconversion photodynamic nanoplatform for an admirable combination therapy of cancer.
其他文献
Depression is a devastating mental disease with high morbidity and mortality rates,intimately linked with oxidative stress.Peroxisomes can produce and scavenge reactive oxygen species(ROS),playing a p
以顺铂为代表的金属抗肿瘤药物与DNA 形成交联或单功能复合物,导致DNA 双螺旋结构扭曲,抑制DNA 复制、转录,最终诱导细胞凋亡或坏死1.为诠释肿瘤细胞如何应答金属药物对DNA 的损伤,进而启动下游的信号转导,我们构建了一种磁性纳米亲和探针,从MCF-7 肿瘤细胞中分离富集1,2-d(GpG)顺铂交联损伤DNA 的应答蛋白质.
As a well-known toxic air pollutant,SO2 displays broad and complex toxicological effects,including oxidative damage,deoxyribonucleic acid(DNA)damage,changes in ion channels and signal transduction as
The development of near-infrared(NIR)fluorescent probe(650-900 nm)has gained increasing attention over the last few decades because of their potential applications in several important fields such as
Effective delivery of probes and drugs to tumor cells remains major challenge for cancer diagnosis and treatment.Nanoparticles capable of escaping the reticuloendothelial system while improving endocy
While most nanomedicine is playing important role in cancer therapy,the nanomaterial may pose a threat to the integrity of the vasculature system.It has been earlier reported that certain nanoparticle
In recent study,SWNHs has a high near-infrared optical absorption capacity,which can effectively convert near-infrared light into heat[1].However,its poor water dispersion and adsorption of plasma pro
碳量子点(CDs)具有荧光发射优异、毒性低和生物相容性良好等优点，但CDs 发射波长短、特异性靶向能力缺乏等不足限制了其在生物成像、治疗等生物领域的实际应用。在此，我们以对氨基偶氮苯为原料一步水热合成了一种具有红色荧光发射的CDs(RCDs)。体外细胞实验表明RCDs 具有较小的细胞毒性，适用于生物研究。共聚焦成像研究进一步揭示RCDs通过与细胞核内RNA 作用有效地靶向到核仁，实现对细胞核的成像
单壁碳纳米角(Single-wall carbon nanohorns,SWNHs)在合成过程中不需使用金属催化剂催化,使其表现出低毒性；同时,其能有效地将近红外光转换成热,是一种理想的纳米光热试剂[1]。然而,水溶性差、靶向性缺乏的不足严重限制了它在生物医学上的实际应用。二氢卟吩e6(Chlorine e6,Ce6)具有高的单线态氧量子产率,可以被用于光动力治疗肿瘤,但其在血液中易被清除的不足限