【摘 要】
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Although considerable clinical attempts on various types of cancers have achieved,photodynamic therapy(PDT)still suffers attenuated therapeutic effects due to the developed resistance of cancer cells.
【机 构】
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College of Chemistry & Environmental Science,Chemical Biology Key Laboratory of Hebei Province,Key L
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Although considerable clinical attempts on various types of cancers have achieved,photodynamic therapy(PDT)still suffers attenuated therapeutic effects due to the developed resistance of cancer cells.As a member of anti-apoptosis protein family,survivin has been demonstrated to be selectively overexpressed in most human malignant tumors and plays a significant role in cancer progression and therapeutic resistance.Herein,we present an upconversion nanoplatform for enhanced PDT by DNAzyme-mediated gene silencing of survivin.In our system,a long single-stranded DNA(ssDNA)with repeated aptamer(AS1411)and DNAzyme was fabricated by rolling circle amplification(RCA)and adsorbed on the upconversion nanoparticles(UCNPs)by the electrostatic attraction.The multivalence of the ssDNA endows the upconversion nanoplatform with high recognition and loading capacity of photosensitizers and DNAzymes.Once the nanoplatform is targeted delivered into cancer cells,the PDT can be activated by near-infrared(NIR)light to generate ROS for killing the cancer cells.And the encoded DNAzyme can efficiently inhibit the expression of survivin gene,allowing the potential to enhance the efficiency of PDT.Our work thus highlights the promise of upconversion photodynamic nanoplatform for an admirable combination therapy of cancer.
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