CIS display is a proprietary acellular in vitro display technology that is capable of displaying very large libraries (>10E13) of polypeptides.Unlike ribosome and mRNA techniques, the library is encoded by a DNA template and the phenotype-genotype link is established by a DNA binding protein that has the unusual characteristic of faithfully binding its own template.We have integrated CIS display with next generation sequencing to provide a powerful tool for the mining of small peptide libraries for a variety of applications.We will present data from this platform to include peptide selection s, maturation and data from the engineering of a small, synthesisable protein domain.