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Adoptive therapy is a promising approach for the treatment of cancers.In adoptive therapy,antigen-presenting cells (APCs) loaded with tumor specific antigens stimulate the proliferation of peripheral blood mononuclear cells (PBMCs) to produce tumor antigen-specific CTLs that are transferred back to patients to kill cancer cells.However,tumor antigen-specific CTLs are difficult to get to tumor tissues,due to immune suppression in patients.Interferon-inducible protein-10 (IP-10),a powerful chemokine,attracts CTLs to tumor tissues and improves their anti-tumor activity.In this study,we developed folate-modified chitosan nanoparticles coating the human IP-10 gene (FA-CS-hIP-10),which bound to the folate receptors on hepatoma cells and promoted the expression of IP-10 to improve the activity of pMAGE-A1278-286 specific CTLs.The combination of FA-CS-hIP-10 and pMAGEA1278- 286 specific CD8+ CTLs increased the secretion of IFN-γ,inhibited tumor growth and extended the survival of nude mice with subcutaneously transplanted human hepatocellular carcinoma.Our results demonstrated that the mechanism of this novel therapy approach was involved in the inhibition of angiogenesis,inhibition of proliferation,and promotion of apoptosis of tumor cells.Our study provided a potentially novel approach for the treatment of human hepatocellular carcinoma by improving the activity of tumor antigen-specific CTLs.