Study on the effects of ATG3 gene over expression by lentivirus on myelodysplastic syndrome cell lin

来源 :22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会) | 被引量 : 0次 | 上传用户：qdmark

【摘要】

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【作者】

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Lin Wang Xiao-Ping Xu Yan Ma Bo-Bin Chen

【机构】

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Department of Hematology,Huashan Hospital,Fudan University,Shanghai,China

【出处】

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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)

【发表日期】

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2013年7期

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Objective: To investigate the effects of ATG3 gene overexpression by lentivirus on SKM-1 cell line and of the ATG3gene on myelodysplastic syndrome to leukemic transformation.Methods: The lentivirus vector was chosen for the delivery of the ATG3 gene plasmid.The SKM-1 cell line was infected with lentivirus vectors.Western blot was performed to detect the ATG3 protein.Real-time PCR was performed to calculate the titer of viruses.The changes in the growth curve, cell vitality, cell apoptosis, and tumor formation in the mice were observed and compared with one another.

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Study on the effects of ATG3 gene over expression by lentivirus on myelodysplastic syndrome cell lin

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