【摘 要】
：
Triptorelin is one of the most common hormone therapies used for prostate cancer.As a peptide drug, the determination of triptorelin using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is
【机 构】
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JOINN Laboratories Inc., Safety Evaluation of Biological Products Beijing Key Laboratory, Beijing 10
【出 处】
：
2015年第一届药代动力学朝阳论坛
论文部分内容阅读
Triptorelin is one of the most common hormone therapies used for prostate cancer.As a peptide drug, the determination of triptorelin using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a daunting task in consideration of the complexity of plasma, which may give rise to a great amount of interference peaks.To solve this problem, a solid-phase extraction (SPE) was developed with protein precipitation for the sample preparation.A 200 μl volume of plasma sample was precipitated with methanol, and then purified with Oasis (R) HLB SPE cartridges;10μl of the eluent was injected into the ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) system.The linear range was 0.01-10 ng/ml, and the limit of quantification (LOQ), was 0.01 ng/ml.This UPLC-MS/MS method was evaluated by specificity, linearity, LOQ, intra-and inter-day precision and accuracy;recovery;and stability.
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