【摘 要】
：
The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371.Its coding region is 1302 bp in length, encoding 433 amino acids, with a predicted protein molecula
【机 构】
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School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China
【出 处】
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The 13th Interational Trichoderma and Gliocladium Workshop &
论文部分内容阅读
The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371.Its coding region is 1302 bp in length, encoding 433 amino acids, with a predicted protein molecular weight of 44.9 kDa and pl of 5.91.Th5545 was shown by RT-qPCR analysis to be differentially transcribed in response to eight different treatments.The transcription of Th5545 was up-regulated when grown in mineral medium, under carbon starvation, and nitrogen starvation, and in the presence of 1％ root powder,1％ stem powder, and 1％ leaf powder derived from Populus davidiana x P.bolleana (Shanxin poplar) aseptic seedlings.The highest increase in transcription approached 3.5-fold that of the control at 6 h under induction with 1％ poplar root powder.
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