Genomic and transcriptomic analysis of hemolysis-relevant mechanisms in Klebsiella pneumoniae after

来源 :中国空间科学学会空间生命专业委员会第二十届学术研讨会暨中国宇航学会航天医学工程与空间生物学专业委员会第四届学术研讨会 | 被引量 : 0次 | 上传用户:xiaoxiao_666
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Various hemolysis-related methods were utilized to explore how Klebsiella pneumoniae acquired hemolytic properties after the Shenzhou Ⅷ spaceflight mission.K.pneumoniae was carried into space by the Shenzhou Ⅷ spacecraft for approximately398 hours.After spaceflight, a mutant strain with hemolytic properties was observed.Genomic and transcriptomic analyses were performed to identify variations;these analyses included comparative genomics, gene expression comparison, dN/dS calculation, and detection of promoters, transcription factor-binding sites and inserted sequences.After spaceflight on the Shenzhou Ⅷ spacecraft, the K.pneumoniae strain LCT-KP182 exhibited significantly higher hemolytic activity compared to the K.pneumoniae strain LCT-KP214, which was cultured on earth and subjected to spaceflight simulation.Alignment of the genome sequences of both strains revealed that most genomic regions were conserved in the strain LCT-KP182;however, in this strain, there were variations in four genes related to hemolysin transport and hemolytic ability.Analysis of gene expression and selection pressure revealed that the toxin TisB was expressed at a higher level in the strain LCT-KP182: therefore, this strain could withstand higher concentrations of hemolysin.dN/dS analysis revealed that the sequence of the gene encoding the hemolysin activator HlyB was conserved in the strain LCT-KP182.Analysis of transcription factor-binding sites revealed that binding site of the transcriptional repressor AbrB upstream of the phospholipase synthesis gene was mutated in strain LCT-KP182, which potentially enhanced hemolysis in this strain.Furthermore, an inserted sequence (IS) in the strain LCT-KP182introduced a gene island related to the Fe3+-siderophore transport system, potentially increasing hemolytic activity in this strain.This study provides various tools for the study of hemolysis in K.pneumoniae and establishes a valuable foundation for research on microorganisms subjected to spaceflight, paving the way for future in vivo simulation studies.
其他文献
表观遗传学是研究在没有细胞核DNA序列改变的情况下,基因功能的可逆的、可遗传的改变.这些改变包括DNA的修饰(如甲基化修饰)、RNA干扰、组蛋白修饰等,这些改变的异常将影响染色质结构和基因的表达,导致复杂综合征、多因素疾病以及癌症.研究已发现,在包括结肠癌、前列腺癌、乳腺癌和血癌在内的许多癌症中,表观遗传变化都是罪魁祸首.表观遗传过程受到饮食、生活方式、环境等外部因素的影响.表观遗传网络作为整合细
美国近年来通过美国国家航空航天局(NASA)内设立的空间生命科学和物理学主管部门和NASA以合同形式授权的公益组织一空间科学研究促进中心(CASIS),双管齐下扩大利用国际空间站开展空间生命科学研究的机会,短期内取得了较大进展.本文介绍在官方、非官方渠道共同推动下的美国ISS上空间生命科学研究的新动向,重点是近年来ISS在空间生命科学领域发布的新项目指南及其项目遴选情况.一、NASA方面按照美国国
在空问站内长期执行任务的宇航员体内会发生严重的骨矿流失问题,空间微重力环境是导致此种骨结构重建的主要因素,但其细胞/分子机制仍不清楚。骨组织的结构重建主要由其内部负责骨形成的成骨细胞和负责骨吸收的破骨细胞来动态调控,前者由骨髓间充质干细胞分化而成,而后者来源于造血干细胞。阐明骨组织细胞的力学生物学机制对于了解微重力环境如何调控以上骨重建过程具有重要意义。成骨或破骨过程分别以钙的沉积或溶解为特征,同
目的:空间环境具备失重、高真空、微磁场和粒子辐射等特点,能够影响微生物的生物学形态和表型改变.本研究借助神舟八号飞船,搭载了肺炎克雷伯菌进入太空进行实验.对回收的肺炎克雷伯菌进行形态学以及比较基因组学的研究,以期发现经过空间飞行后肺炎克雷伯菌的变化及其机制.方法:对返回地面的肺炎克雷伯菌株进行16s测序鉴定菌株.革兰氏染色,光镜下观察肺炎克雷伯菌形态学变化.对空间飞行肺炎克雷伯菌株和地面对照株进行
会议
对搭载于神舟八号飞船14天空间飞行的屎肠球菌进行突变株筛选,并鉴定和分析差异表达的蛋白质。利用Biolog生化反应板从搭载神舟八号飞船飞行后屎肠球菌中筛选突变株,提取突变株蛋白质并进行SDS-PAGE电泳检测,用BCA法测定总蛋白浓度后,采用同重同位素相对与绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)方法分析空间环
NDM-1基因是近年来爆发的耐碳氢青霉烯类抗生素的多耐药基因,而携带此基因的肺炎克雷伯杆菌也在全球多地被发现,对人类的健康构成了极大的威胁。在本实验中,我们通过神州10号飞船将NDM-1肺炎克雷伯杆菌(ATCCBAA-2146)送入太空环境中培养后,进一步研究太空环境对此细菌环境适应性的影响。与此同时,我们通过微重力模拟器模拟微重力环境并同时以重力环境下培养的此细菌作为对照组。研究结果表明:在太空
空间的极端环境可以影响微生物的行为,进而可能增加太空飞行中发生感染性疾病的风险.然而,甲氧西林耐药金黄色葡萄球菌(MRSA)对太空飞行环境下反应的分子遗传学基础仍未能充分阐释.在本项研究中,我们对一株从临床患者分离的MRSA母株(LCT-SAO),以及在神舟十号飞船搭载(LCT-SAS)、地面模拟微重力(LCT-SAM)和地面静止(LCT-SAG)三种不同培养条件下的MRSA菌株分别进行了基因组测
Introduction The greatest exposure to static magnetic flied (SMF) is geomagnetic field (GMF),with the strength approximately 20~70 μT.By using permanent magnets and superconducting technology, human h
Introduction It is well-known that gravitropism, one of the important physiological phenomenon, is the plant growth movement in response to gravity.Typically, it allows plant root grows toward the dir
To explore the effects of space on Klebsiella pneumoniae after spaceflights.A strain of Klebsiella pneumoniae was sent to space for 398 hours by ShenZhouⅧ during November 1, 2011, to November 17, 2011