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Paclitaxel (PTX),a chemotherapeutic drug,affects microtubule dynamics and influences endocytic trafficking.However,the mechanism and the dynamics of altered endocytic trafficking by paclitaxel treatment in single living cells still remain elusive.By labeling quantum dots (QDs) to the epidermal growth factor (EGF),we continuously tracked the endocytosis and post-endocytic trafficking of EGF receptors (EGFRs) in A549 cells for a long time interval.A single-cell analysis method was introduced to quantitatively study the dynamics of endocytic trafficking.Compared with the control cells,the velocity of directed motion was reduced by 30% due to the suppression of high speed movements of EGF-QDs along the microtubules in PTX-treated cells.