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To explore a new 6-substituted naphthalimide derivatives compound (3b14) in vitro inhibitsLung cancer cell proliferation activity and mechanism.we measured the inhibitive effectof compound (3b14) on human lung cancer cell line A549,NCI-H460, and NCI-H446,meanwhile tested the toxicity of human umbilical vein endothelial cell line HUVEC by MTTassay; The effect on NCI-H460 cells cell cycle is detected by Flow cytometry; We use thefluorescent staining to observe the NCI-H460 cells nucleus morphological changes ofapoptotic, and if the apoptosis inducing factor (AIF) can transfer into the nucleus to induceapoptosis by cell immunohistochemistry; Western blot method detects "p16-CyclinD1-CDK4-Rb" key cell cycle control pathways in NCI-H460 cells.