【摘 要】
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@@In this paper, a poly(dimethylsiloxane) (PDMS) microchannel was used for electrophoretic separation of neurotransmitters. For promotion of the separation efficiency, the microchannel was modified by
【机 构】
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Institute of Analytical Chemistry, Department of Chemistry, Dushu Lake Campus, Suzhou University, Su
【出 处】
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第一届国际微纳尺度生物医学分离和分析技术学术会议暨第六届全国微全分析学术会议
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@@In this paper, a poly(dimethylsiloxane) (PDMS) microchannel was used for electrophoretic separation of neurotransmitters. For promotion of the separation efficiency, the microchannel was modified by polystyrene sulphonate (PSS) / polystyrene nano-sphere (PSNS) self-assembled layer. The quasi-ordered monolayer of PSNS was firstly transferred onto the wall of PDMS channel from the air/water interface by draining method. Then the PSS was adsorbed onto its surface to form the modified layer. The PSNS / PSS modified PDMS microchannel exhibited lower, stable EOF and higher separation efficiency. Under optimized conditions, a group of neurotransmitters (5-hydroxytryptamine, 5-HT; dopamine, DA; epinephrine, EP; catechol, CA) were excellently separated in a 3.5 cm length separation channel and successfully amperometric detected with a carbon micro-disk electrode which was located opposite on outlet of microchannel. Compared with the native PDMS and poly(dimethylammoniumchloride) (PDDA) / PSS modified one, the proposed modifying methodology showed prominent higher resolution (R) for their separation.
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