【摘 要】
：
目的 聚羟基脂肪酸酯(PHA)是一类具有良好生物相容性的可降解高分子材料,3-羟基丁酸和3-羟基己酸共聚酯(PHBHHx)是PHA家族中的一员,其性能优于PHA家族的其它材料.研究表明PHBHHx对体外贴壁生长的成骨细胞、软骨细胞、成纤维细胞等多种细胞具有良好的生物相容性,但是对于和体外悬浮生长的神经干细胞(NSC)的相容性欠佳.本研究拟对PHBHHx膜进行表面改性,以增强与NSC的组织相容性,进
【机 构】
：
西安交通大学医学院神经生物学研究所,西安710061 西安交通大学生命学院,西安710049
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
目的 聚羟基脂肪酸酯(PHA)是一类具有良好生物相容性的可降解高分子材料,3-羟基丁酸和3-羟基己酸共聚酯(PHBHHx)是PHA家族中的一员,其性能优于PHA家族的其它材料.研究表明PHBHHx对体外贴壁生长的成骨细胞、软骨细胞、成纤维细胞等多种细胞具有良好的生物相容性,但是对于和体外悬浮生长的神经干细胞(NSC)的相容性欠佳.本研究拟对PHBHHx膜进行表面改性,以增强与NSC的组织相容性,进而为以NSC为基础的脑组织工程应用奠定基础.方法 通过溶剂挥发法制备PHBHHx的二维膜支架,扫描电镜观察其表面性状；分别通过脂肪酶处理,NaOH处理和等离子处理的方法对PHBHHx膜进行表面改性,测量接触角大小检测膜表面亲水性；分离孕14.5天大鼠胚胎大脑皮质NSC,培养于NSC完全培养基(培养基成分：无血清的DMEM/F 12+N2+B27+EGF+bFGF)；培养至第三代的NSC分别接种在未处理、脂肪酶处理、碱处理、等离子处理以及碱+脂肪酶处理的PHBHHx支架膜表面,免疫染色观察细胞的分布状态,CCK-8试剂盒检测细胞生长状态,免疫细胞荧光化学观察NSC分化情况.结果 与未处理的PHBHHx膜相比,脂肪酶+NaOH联合处理、等离子处理后,接触角明显减小,提示上述处理可以增强PHBHHx膜表面的亲水性；NSC在接种于PHBHHx支架后,能够继续存活,细胞形态饱满、折光性强,与未做表面改性处理组相比,处理后随着膜亲水性的增强,表面贴附细胞数量增加,存活状态良好,而且能够发生自发分化,分化为神经元和胶质细胞；与PHBHHx支架的共培养促进了NSC的增殖.结论 PHBHHx材料表面亲水性增强可以提高与NSC的组织相容性；与PHHBx材料的共培养,不改变NSC原有的增殖和多向分化潜能.
其他文献
Objective Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system.A growing body of evidence suggests that water channel aquaporin-
Objective Several lines of evidences indicated that autophagy was involved in ischemic brain injury.Ischemia/reperfusion injury may lead to autophagic cell death, while some studies also suggested tha
Objective Overexpression of glial fibrillary acidic protein (GFAP) is a hallmark of astrogliosis induced by injury and disease in the central nervous system.The mechanism of GFAP upregulation in react
Chemokines are implicated in neuroinflammation of several chronic neurodegenerative disorders.However, the precise role of chemokines in neurodegeneration is unknown.Thiamine deficiency (TD) causes ab
Huntingtons disease (HD) is a familial neurodegenerative disorder caused by an aberrant expansion of polyglutamine in the N-terminal region of huntingtin (Htt) and is characterized by restricted neuro
Objective To observe the peripheral nerve regeneration of injured sciatic nerve after adenovirus-mediated gene transfer of brain-derived neurotrophic factor (BDNF) in adult rats.Methods Based on silic
Objective In focal cortical dysplasia type Ⅱb (FCDⅡb) and tuberous sclerosis complex (TSC), malformative cortical lesions in the cerebral cortex cause epilepsy and other neurological abnormalities.How
Objective Polyphosphate (polyP) was widely studied in microorganism as potential high-energy phosphate source and stringent response-related regulatory factor.It was discovered in mammalian brain by A
Objective This study was to identify Proteolipid protein 1 (PLP1) mutations in 33 unrelated Chinese patients (P1-33) with Pelizaeus-Merzbacher disease (PMD) and prenatal diagnosis of 5 fetuses in 3 Ch
Objective To clarify whether the pathologic events of HIV encephalitis and AIDS dementia occur independently in human HIV-1 infection.Methods Twenty brains of patients with HIV-1 infection autopsied i