Because of the cerebral infarctions characteristic of high incidence,high morbidity,recurrence rate,high mortality and higher health care costs,it has become a hot research field of science and medicine,and animal models plays an important role in basic research and preclinical drug evaluation of cerebral infarction.Nonhuman primates as human kin,is very similar to human in morphology,physiology,biochemistry and metabolism.So Nonhuman primate model of cerebral infarction is increasingly being recognized and used by the industry.There are many methods to make cerebral infarction model,such as suture method,photochemical method and balloon embolization.But these methods are easy restricted to hardware in the implementation process.The neurosurgery method to make NHP MCAO is simple and easy because the different range of middle cerebral artery can be separated and exposed and blocked under the microscope,such as transorbitalapproach,trans-pterional approach and supraorbital approach to make NHP MCAO.Transorbital approach need to removal of the eye,wear open orbital wall and expose middle cerebral artery.Its advantages are: 1.Simple,fast exposure and blocking the beginning part of the middle cerebral artery; 2.shorter time; 3.no need much microsurgical skills.But its drawback is also very prominent: 1.This surgery required removal of the eye,worn skull base,devastating large,poor animal welfare; 2.Prone to leakage of cerebrospinal fluid,intracranial infections and other complications,the mortality rate is high; 3 after animals completely missing the visual side,the larger impact of subsequent experiments on animals.Therefore,it is essential to carry out better methods to substitute transorbital approach.Supraorbital approach can be exposed directly blocking the initial portion of the middle cerebral artery and achieved with the same orbital path through the effect of surgery,to avoid shortage of the latter,but this method requires a skilled surgeon with neurosurgery surgical skills.Trans-pterional approach can expose various segments of middle cerebral artery in the brain,to meet the different types of MCAO models,and can effectively avoid a sharp rise in intracranial pressure by removing bone after operation and reduce mortality.Therefore,Trans-pterional approach is the best choice for preparation the selective MCA segment occlusion of NHP.